Purification, crystallization and preliminary X-ray diffraction analysis of human phosphoserine phosphatase

被引：9

作者：

Peeraer, Y

Rabijns, A

Verboven, C

Collet, JF

Van Schaftingen, E

De Ranter, C

机构：

[1] Katholieke Univ Leuven, Fac Pharmaceut Sci, Lab Analyt Chem & Med Physicochem, B-3000 Louvain, Belgium

[2] Catholic Univ Louvain, Christian de Duve Inst Cellular Pathol, Physiol Chem Lab, B-1200 Brussels, Belgium

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2002年 / 58卷

关键词：

D O I：

10.1107/S0907444901017310

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphoserine phosphatase (PSP), a human enzyme involved in the l-serine biosynthesis pathway, has been crystallized using the hanging-drop vapour-diffusion method at 277 K. The crystals are orthorhombic, belonging to space group C222(1), with unit-cell parameters a = 49.03 Angstrom, b = 130.25 Angstrom, c = 157.29 Angstrom. Calculation of the Matthews coefficient indicates that there are two molecules in the asymmetric unit. A complete native data set to a resolution of 1.53 Angstrom has been collected at 100 K using synchrotron radiation.

引用

页码：133 / 134

页数：2

共 17 条

[1] The catalytic domain of the P-type ATPase has the haloacid dehalogenase fold
Aravind, L
Galperin, MY
Koonin, EV
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1998, 23 (04) : 127 - 129
[2] Mechanistic studies of phosphoserine phosphatase, an enzyme related to P-type ATPases
Collet, JF
Stroobant, V
Van Schaftingen, E
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (48) : 33985 - 33990
[3] A new class of phosphotransferases phosphorylated on an aspartate residue in an amino-terminal DXDX(T/V) motif
Collet, JF
Stroobant, V
Pirard, M
Delpierre, G
Van Schaftingen, E
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (23) : 14107 - 14112
[4] Human L-3-phosphoserine phosphatase: Sequence, expression and evidence for a phosphoenzyme intermediate
Collet, JF
Gerin, I
Rider, MH
VeigadaCunha, M
VanSchaftingen, E
[J]. FEBS LETTERS, 1997, 408 (03) : 281 - 284
[5] Improved R-factors for diffraction data analysis in macromolecular crystallography
Diederichs, K
Karplus, PA
[J]. NATURE STRUCTURAL BIOLOGY, 1997, 4 (04): : 269 - 275
[6] Diedrichs K, 1997, NAT STRUCT BIOL, V4, P592
[7] Crystal structure of L-2-haloacid dehalogenase from Pseudomonas sp YL - An alpha/beta hydrolase structure that is different from the alpha/beta hydrolase fold
Hisano, T
Hata, Y
Fujii, T
Liu, JQ
Kurihara, T
Esaki, N
Soda, K
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (34) : 20322 - 20330
[8] SPARSE-MATRIX SAMPLING - A SCREENING METHOD FOR CRYSTALLIZATION OF PROTEINS
JANCARIK, J
KIM, SH
[J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 : 409 - 411
[9] COMPUTER-ANALYSIS OF BACTERIAL HALOACID DEHALOGENASES DEFINES A LARGE SUPERFAMILY OF HYDROLASES WITH DIVERSE SPECIFICITY - APPLICATION OF AN ITERATIVE APPROACH TO DATABASE SEARCH
KOONIN, EV
TATUSOV, RL
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1994, 244 (01) : 125 - 132
[10] LINGREL JB, 1994, J BIOL CHEM, V269, P19659

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