Endonuclein is a cell cycle regulated WD-repeat protein that is up-regulated in adenocarcinoma of the pancreas

被引:21
作者
Honore, B
Baandrup, U
Nielsen, S
Vorum, H
机构
[1] Aarhus Univ, Dept Med Biochem, DK-8000 Aarhus C, Denmark
[2] Aarhus Univ, Inst Anat, Dept Cell Biol, DK-8000 Aarhus, Denmark
[3] Aarhus Univ Hosp, Dept Pathol, DK-8000 Aarhus C, Denmark
关键词
cell cycle regulated protein; nucleus; endoplasmic reticulum; signal transduction; chaperone activity;
D O I
10.1038/sj.onc.1205186
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The transcript encoding endonuclein, the human homolog of yeast PWP1, was previously found up-regulated in pancreatic cancer tissue. By immunohistochemistry we detected a ubiquitous presence in several tissues examined: skin, liver, thyroid gland, heart muscle, neurons, kidney, bladder, pancreas, adrenal gland, ovary, uterus, testis and prostate gland. We especially noticed that normal pancreatic exocrine cells exhibited low protein levels while pancreatic adenocarcinoma cells revealed high levels. We found a heterogeneous subcellular distribution, especially with varying nuclear levels. In proliferating cells endonuclein protein expression and localization was cell cycle dependent, with increasing levels and nuclear focusing during the interphase toward mitosis. Ultrastructural analysis revealed ER and nuclear localization. Endonuclein contains five WD-repeats, indicating a putative role in crucial regulatory activities in the nucleus as well as in the ER.
引用
收藏
页码:1123 / 1129
页数:7
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