Protein detection based on microelectrodes with the PPy[3,3-Co(1,2-C2B9H11)]2 solid contact and immobilized proteinases:: Preliminary investigations

被引：13

作者：

Biloivan, OA

Verevka, SV

Dzyadevych, SV

Jaffrezic-Renault, N

Zine, N

Bausells, J

Samitier, J

Errachid, A

机构：

[1] Natl Acad Sci Ukraine, Inst Mol Biol & Genet, Lab Biomol Elect, UA-03143 Kiev, Ukraine

[2] Natl Acad Sci Ukraine, AV Palladin Inst Biochem, Dept Prot Struct & Funct, UA-01030 Kiev, Ukraine

[3] Ecole Cent Lyon, CNRS, UMR 5005, CEGELY, F-69134 Ecully, France

[4] CSIC, IMB, Ctr Nacl Microelect, E-08193 Barcelona, Spain

[5] NanoBioEngn Res Lab, CREBEC, E-08028 Barcelona, Spain

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2006年 / 26卷 / 2-3期

关键词：

microbiosensor; immobilized proteinase; protein; solid-contact ion-selective microelectrodes (SCISME);

D O I：

10.1016/j.msec.2005.10.011

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Protein selective potentiometric microelectrodes with the PPy[3,3-Co(1,2-C2B9H11)(2) solid internal contact were fabricated by the immobilization of different proteinases such as trypsin, pronase E and carboxypeptidase B on the surface of platinum microelectrodes modified by a conducting polymer. The performance of these biosensors was investigated by using potentionnetric measurements. The feasibility of this biosensor for protein determination was demonstrated, with a detection limit of about 1-2 mu g/ml for prolamine in buffer solution. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：574 / 577

页数：4

共 9 条

[1] Biloivan O. A., 1997, Ukrainskii Biokhimicheskii Zhurnal, V69, P14
[2] Potentiometric ion sensors based on conducting polymers
Bobacka, J
Ivaska, A
Lewenstam, A
[J]. ELECTROANALYSIS, 2003, 15 (5-6) : 366 - 374
[3] Cheronis J.C., 1993, PROTEASE PROTEASE IN
[4] Biomolecule immobilization on electrode surfaces by entrapment or attachment to electrochemically polymerized films. A review
Cosnier, S
[J]. BIOSENSORS & BIOELECTRONICS, 1999, 14 (05) : 443 - 456
[5] Enzyme biosensor for tomatine detection in tomatoes
Dzyadevych, SV
Arkhypova, VN
Soldatkin, AP
El'skaya, AV
Martelet, C
Jaffrezic-Renault, N
[J]. ANALYTICAL LETTERS, 2004, 37 (08) : 1611 - 1624
[6] Detection of protease activity in the wetted surface of gelatin-coated electrodes in humid air by AC impedance spectroscopy (vol 15, pg 305, 2000)
Saum, AGE
Cumming, RH
Rowell, FJ
[J]. BIOSENSORS & BIOELECTRONICS, 2000, 15 (11-12) : 693 - 693
[7] WEETALL HH, 1977, BIOTECHNOLOGICAL APP, P109
[8] PROTAMINE-SENSITIVE POLYMER MEMBRANE-ELECTRODE - CHARACTERIZATION AND BIOANALYTICAL APPLICATIONS
YUN, JH
MEYERHOFF, ME
YANG, VC
[J]. ANALYTICAL BIOCHEMISTRY, 1995, 224 (01) : 212 - 220
[9] Hydrogen-selective microelectrodes based on silicon needles
Zine, N
Bausells, J
Ivorra, A
Aguiló, J
Zabala, M
Teixidor, F
Masalles, C
Viñas, C
Errachid, A
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2003, 91 (1-3) : 76 - 82

← 1 →