Development of a locus-specific marker and localization of the Rysto gene based on linkage to a catalase gene on chromosome XII in the tetraploid potato genome

被引：14

作者：

Cernak, Istvan ^{[1
]}

Decsi, Kincso ^{[2
]}

Nagy, Sandor ^{[3
]}

Wolf, Istvan ^{[1
]}

Polgar, Zsolt ^{[1
]}

Gulyas, Gergely ^{[4
]}

Hirata, Yutaka ^{[5
]}

Taller, Janos ^{[2
]}

机构：

[1] Univ Pannonia, Ctr Agr Sci, Potato Res Ctr, H-8360 Keszthely, Hungary

[2] Univ Pannonia, Dept Plant Sci & Biotechnol, Georgikon Fac Agr, H-8360 Keszthely, Hungary

[3] Univ Pannonia, Dept Econ Methodol, Georgikon Fac Agr, H-8360 Keszthely, Hungary

[4] Tokyo Univ Agr & Technol, United Grad Sch Agr Sci, Fuchu, Tokyo 1838509, Japan

[5] Tokyo Univ Agr & Technol, Grad Sch Agr, Fuchu, Tokyo 1838509, Japan

来源：

BREEDING SCIENCE | 2008年 / 58卷 / 03期

基金：

匈牙利科学研究基金会;

关键词：

PVY extreme resistance; Ry(sto) gene; SCAR marker; mapping; intron-targeting method; catalase gene; anchor marker;

D O I：

10.1270/jsbbs.58.309

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 [作物学];

摘要：

As a result of many decades of potato breeding in Keszthely, Hungary, numerous varieties resistant to the pathogens attacking potato have been developed using wild Solanum species as resistance sources. In order to simplify the time-consuming breeding process, the present research project involved the development of a SCAR marker correlating with the Ry(sto) gene, originating from the Solanum stoloniferun species and conferring extreme resistance to the potato Y virus (PVY), using sequence information from a RAPD marker linked to the gene. The usefulness of the marker was tested on 21 varieties resistant or susceptible to PVY and it was found to provide efficient identification of genotypes carrying the Ry(sto) gene. Based on its linkage to a new chromosome-specific anchor marker (Cat-in2), identified by means of the intron-targeting method not previously used in potato research, the Ry(sto) gene was localized on chromosome XII.

引用

页码：309 / 314

页数：6

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