High-performance liquid chromatographic determination of phenylureas by photochemically-induced fluorescence detection

被引：24

作者：

de la Peña, AM ^{[1
]}

Mahedero, MC ^{[1
]}

Bautista-Sánchez, A ^{[1
]}

机构：

[1] Univ Extremadura, Fac Sci, Dept Analyt Chem, Badajoz 06071, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 950卷 / 1-2期

关键词：

photochemically induced fluorescence detection; fluorescence detection; detection; LC; pesticides; phenylureas;

D O I：

10.1016/S0021-9673(02)00042-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A HPLC method, using photochemically-induced fluorescence detection, is described for the separation and determination of four phenylurea herbicides including diuron, isoproturon, linuron and neburon. A post-column photoreactor, consisting of a reactor knitted around a 4 W xenon lamp, has been included between the column and the detector, in order to transform the non-fluorescent herbicides into fluorophors. The influence of mobile phase composition, flow-rate, pH, and buffer concentration has been studied. An acetonitrile-buffer solution of potassium phosphate dibasic of pH 7 and 0.01 M concentration (60:40, v/v), was selected as optimum. For the fluorimetric detection, optimal excitation/emission wavelengths 324/403, 301/433, 335/411 and 326/385 nm were selected for the determination of diuron, isoproturon, linuron and neburon, respectively. The detection limits ranged between 0.07 and 0.46 mug/ml, according to the compound. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：287 / 291

页数：5

共 10 条

[1] Usefulness of micellar media for the quantitative analysis of phenylurea herbicides in water by photochemically-induced fluorescence [J].