Energy transfer in aqueous solutions of oppositely charged CdSe/ZnS core/shell quantum dots and in quantum dot-nanogold assemblies

被引:205
作者
Wargnier, R
Baranov, AV
Maslov, VG
Stsiapura, V
Artemyev, M
Pluot, M
Sukhanova, A
Nabiev, I
机构
[1] Univ Reims, Inst Federatif Rech Biomol 53, EA Interact Cellulaires & Mol Cancerol 3306, F-51100 Reims, France
[2] Belarusian State Univ, Inst Physicochem Problems, Minsk 220080, BELARUS
[3] SI Vavilov State Opt Inst, St Petersburg 199034, Russia
关键词
D O I
10.1021/nl0350938
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We demonstrate that the nanoscale assemblies of water-solubilized, oppositely charged CdSe/ZnS core/shell quantum dots and of quantum dots and nanogold particles make it possible to develop FRET-based sensors with a donor quenching efficiency close to 100%. The cw and time-resolved photoluminescence analysis of nanoassemblies with different donor-to-acceptor ratios demonstrates a prevalence of 1:1 complexes. Experimental data perfectly fit the mathematical simulation based on long-range FRET and a static model of the assembling of the oppositely charged nanoparticles. These assemblies are stable in biological fluids, suggesting new applications.
引用
收藏
页码:451 / 457
页数:7
相关论文
共 25 条
[1]  
Agranovich V.M., 1982, Electronic Excitation Energy Transfer in Condensed Matter
[2]   Nanocrystal targeting in vivo [J].
Åkerman, ME ;
Chan, WCW ;
Laakkonen, P ;
Bhatia, SN ;
Ruoslahti, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) :12617-12621
[3]   Effect of ZnS shell thickness on the phonon spectra in CdSe quantum dots [J].
Baranov, AV ;
Rakovich, YP ;
Donegan, JF ;
Perova, TS ;
Moore, RA ;
Talapin, DV ;
Rogach, AL ;
Masumoto, Y ;
Nabiev, I .
PHYSICAL REVIEW B, 2003, 68 (16)
[4]   In vivo imaging of quantum dots encapsulated in phospholipid micelles [J].
Dubertret, B ;
Skourides, P ;
Norris, DJ ;
Noireaux, V ;
Brivanlou, AH ;
Libchaber, A .
SCIENCE, 2002, 298 (5599) :1759-1762
[5]   Conjugation of luminescent quantum dots with antibodies using an engineered adaptor protein to provide new reagents for fluoroimmunoassays [J].
Goldman, ER ;
Anderson, GP ;
Tran, PT ;
Mattoussi, H ;
Charles, PT ;
Mauro, JM .
ANALYTICAL CHEMISTRY, 2002, 74 (04) :841-847
[6]   Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules [J].
Han, MY ;
Gao, XH ;
Su, JZ ;
Nie, S .
NATURE BIOTECHNOLOGY, 2001, 19 (07) :631-635
[7]   Electronic energy transfer in CdSe quantum dot solids [J].
Kagan, CR ;
Murray, CB ;
Nirmal, M ;
Bawendi, MG .
PHYSICAL REVIEW LETTERS, 1996, 76 (09) :1517-1520
[8]   Long-range resonance transfer of electronic excitations in close-packed CdSe quantum-dot solids [J].
Kagan, CR ;
Murray, CB ;
Bawendi, MG .
PHYSICAL REVIEW B, 1996, 54 (12) :8633-8643
[9]   APPLICATION OF THE SPECTROSCOPIC RULER TO STUDIES OF THE DIMENSIONS OF FLEXIBLE MACROMOLECULES .1. THEORY [J].
LIU, GJ ;
GUILLET, JE .
MACROMOLECULES, 1990, 23 (05) :1388-1392
[10]  
MARSHALL AG, 1978, BIOPHYSICAL CHEM PRI