Autocrine activation of cultured macrophages by brain-derived neurotrophic factor

被引：39

作者：

Asami, Toshio ^{[1
]}

Ito, Takuya ^{[1
]}

Fukumitsu, Hidefumi ^{[1
]}

Nomoto, Hiroshi ^{[1
]}

Furukawa, Yoshiko ^{[1
]}

Furukawa, Shoei ^{[1
]}

机构：

[1] Gifu Pharmaceut Univ, Mol Biol Lab, Gifu 5028585, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 344卷 / 03期

关键词：

macrophages; culture; pliagocytic activity; brain-derived neurotrophic factor (BDNF); TrkB; Neurotrophin-3 (NT-3); lipopolysaccharide (LPS);

D O I：

10.1016/j.bbrc.2006.03.228

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To elucidate a significance of the expression of brain-derived neurotrophic factor (BDNF) in the activated microglia/macrophages of the injured central nervous system. we examined BDNF actions on or BDNF synthesis by macrophages Cultured from the mouse peritoneal cavity. They synthesized BDNF and neurotrophin-3 (NT-3) in addition to expressing high-affinity neutrophin receptors, fulll-length TrkB (FL), truncated TrkB (TK-), and TrkC, thus suggesting an autocrine influence of BDNF Mid NT-3. BDNF. but not NT-3. enhanced phagocytic activity and stimulated synthesis/secretion of interleukin-1 beta in the same manner as lipopolysaccharide ( LPS). Furthermore. there was a significant correlation of the phagocytic activity with the expression of BDNF or TrkB (FL). These results imply that the phagocytic activity of macrophages depends on BDNF synthesis and/or TrkB (FL expression, suggesting that BDNF participates in the activation processes of macrophages by acting in an autocrine manner. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：941 / 947

页数：7

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