Structural determination of the O-antigenic polysaccharide from the Shiga toxin-producing Escherichia coli O171

被引：21

作者：

Ali, Tara

Weintraub, Andrej

Widmalm, Gran ^{[1
]}

机构：

[1] Stockholm Univ, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden

[2] Karolinska Univ Hosp, Karolinska Inst, Div Clin Bacteriol, Dept Lab Med, S-14186 Huddinge, Sweden

来源：

CARBOHYDRATE RESEARCH | 2006年 / 341卷 / 11期

关键词：

Escherichia coli; verotoxin; lipopolysaccharide; sialic acid; NMR;

D O I：

10.1016/j.carres.2006.04.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the O-antigenic part of the lipopolysaccharide (LPS) obtained from the verotoxin-producing Escherichia coli O171 has been determined. H-1 and C-13 NMR spectroscopy techniques in combination with component analysis were used to elucidate the O-antigen structure of O-deacylated LPS. Subsequent NMR analysis of the native LPS revealed acetylation at O-7/O-9 of the sialic acid residue. The sequence of sugars was determined by inter-residue correlations in H-1,H-1-NOESY and H-1, C-13-heteronuclear multiple-bond correlation spectra. The O-antigen is composed of pentasaccharide repeating units with one equivalent of O-acetyl groups distributed over two positions: -> 4)-alpha-Neu5Ac7,9Ac-(2 -> 6)-beta-D-Galp-(1 -> 6)-p-D-Galp-(1 -> 6)-beta-D-Galp-(1 -> 3)-beta-D-GalpNAc-(1 -> Based on biosynthetic considerations, this should also be the biological repeating unit. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：1878 / 1883

页数：6

共 40 条

[1] Structural studies of the O-antigenic polysaccharides from the enteroaggregative Escherichia coli strain 522/C1 and the international type strain from Escherichia coli O178 [J].