Lineage tracing with Axin2 reveals distinct developmental and adult populations of Wnt/β-catenin-responsive neural stem cells

被引:75
作者
Bowman, Angela N. [1 ,3 ]
van Amerongen, Renee [1 ,3 ]
Palmer, Theo D. [2 ]
Nusse, Roeland [1 ,3 ]
机构
[1] Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Neurosci, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA
[3] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA
基金
美国国家科学基金会;
关键词
radial glia cell; astrocyte; forebrain; RADIAL GLIA; SUBVENTRICULAR ZONE; SONIC HEDGEHOG; MAMMALIAN HIPPOCAMPUS; CEREBRAL-CORTEX; NERVOUS-SYSTEM; OLFACTORY-BULB; IN-VIVO; BRAIN; NEUROGENESIS;
D O I
10.1073/pnas.1305411110
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Since the discovery of neural stem cells in the mammalian brain, there has been significant interest in understanding their contribution to tissue homeostasis at both the cellular and molecular level. Wnt/beta-catenin signaling is crucial for development of the central nervous system and has been implicated in stem cell maintenance in multiple tissues. Based on this, we hypothesized that the Wnt pathway likely controls neural stem cell maintenance and differentiation along the entire developmental continuum. To test this, we performed lineage tracing experiments using the recently developed tamoxifen-inducible Cre at Axin2 mouse strain to follow the developmental fate of Wnt/beta-catenin-responsive cells in both the embryonic and postnatal mouse brain. From as early as embryonic day 8.5 onwards, Axin2(+) cells can give rise to spatially and functionally restricted populations of adult neural stem cells in the subventricular zone. Similarly, progeny from Axin2(+) cells labeled from E12.5 contribute to both the subventricular zone and the dentate gyrus of the hippocampus. Labeling in the postnatal brain, in turn, demonstrates the persistence of long-lived, Wnt/beta-catenin-responsive stem cells in both of these sites. These results demonstrate the continued importance of Wnt/beta-catenin signaling for neural stem and progenitor cell formation and function throughout developmental time.
引用
收藏
页码:7324 / 7329
页数:6
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