Nitrile hydratase from Mesorhizobium sp F28 and its potential for nitrile biotransformation

被引：24

作者：

Feng, Yun-Shu ^{[1
]}

Chen, Pei-Chung ^{[2
]}

Wen, Fu-Shyan ^{[3
]}

Hsiao, Wan-Yi ^{[3
]}

Lee, Chi-Mei ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Dept Environm Engn, Taichung 402, Taiwan

[2] Hungkuang Univ, Inst Clin Nutr, Taichung 433, Taiwan

[3] Natl Chung Hsing Univ, Dept Life Sci, Taichung 402, Taiwan

来源：

PROCESS BIOCHEMISTRY | 2008年 / 43卷 / 12期

关键词：

Acrylamide; Acrylonitrile; Biotransformation; Mesorhizobium sp; Nitrile; Nitrile hydratase;

D O I：

10.1016/j.procbio.2008.08.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mesorhizobium sp. F28 was isolated from a wastewater treatment system in a polyacrylonitrile (PAN) fibre factory for its ability to convert acrylonitrile by a nitrile hydratase (NHase)-amidase (AMase) system. This strain completely degraded acrylonitrile at concentrations as high as 18.4 mM, and exhibited 192 U mg(-1) dcw NHase activity for the conversion of acrylonitrile to acrylamide. The cobalt-containing NHase purified from the strain F28 was moderately thermostable, keeping full activity up to 45 C; it also had Optimum activity between pH 7.0 and 7.5. Acrylonitrile, propionitrile, isobutyronitrile, and benzonitrile were good Substrates for the NHase of Mesorhizobium sp. F28. The affinity of the NHase for acrylonitrile (K-m = 0.53 mM) was greater than that of bacteria mentioned by other researchers before. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1391 / 1397

页数：7

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