Duhuo Jisheng Decoction promotes chondrocyte proliferation through accelerated G1/S transition in osteoarthritis

被引：49

作者：

Wu, Guangwen ^{[1
]}

Chen, Wenlie ^{[1
]}

Fan, Huailing ^{[2
]}

Zheng, Chunsong ^{[1
]}

Chu, Jianfeng ^{[1
]}

Lin, Ruhui ^{[3
]}

Ye, Jinxia ^{[3
]}

Xu, Huifeng ^{[1
]}

Li, Xihai ^{[1
]}

Huang, Yunmei ^{[3
]}

Ye, Hongzhi ^{[1
]}

Liu, Xianxiang ^{[1
]}

Wu, Mingxia ^{[4
]}

机构：

[1] Fujian Univ Tradit Chinese Med, Acad Integrat Med, Biomed Res Ctr, Fuzhou 350122, Fujian, Peoples R China

[2] Fujian Univ Tradit Chinese Med, Acad Tradit Chinese Med, Biomed Res Ctr, Fuzhou 350122, Fujian, Peoples R China

[3] Fujian Univ Tradit Chinese Med, Acad Integrat Med, Biomed Res Ctr, Fuzhou 350122, Fujian, Peoples R China

[4] Fujian Univ Tradit Chinese Med, Affiliated Peoples Hosp 2, Fuzhou 350003, Fujian, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2013年 / 32卷 / 05期

关键词：

Duhuo Jisheng Decoction; chondrocyte; proliferation; cell cycle; osteoarthritis; CELL-CYCLE; IN-VITRO; ARTICULAR CHONDROCYTES; CARTILAGE; EXPRESSION; CANCER; PHOSPHORYLATION; DEGRADATION; P16(INK4A);

D O I：

10.3892/ijmm.2013.1481

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Duhuo Jisheng Decoction (DHJSD), a well known traditional Chinese folk medicine, is used for eliminating stagnation, removing blood stasis, promoting blood circulation and alleviating pain; it is commonly used for the treatment of various diseases, including osteoarthritis (OA). However, the molecular mechanisms behind the therapeutic effects of OA remain unclear. In the present study, the effects of DHJSD on the morphology of articular cartilage and the G1/S cell cycle progression in chondrocytes, as well as the underlying mechanisms, were investigated. A total of 27 two-month-old male Sprague Dawley rats were randomly divided into 3 groups: the control group (no papain-induced OA; received an equivalent amount of saline only), the model group (papain-induced OA; received an equivalent amount of saline only) and the DHJSD group [papain-induced OA; received a clinical oral dose of DHJSD (9.3 g/kg/day)]. After 8 consecutive weeks of treatment, the morphological changes in articular cartilage were observed under an optical microscope and by transmission electron microscopy (TEM) and the mRNA and protein expression levels of cyclin D1, CDK4, CDK6, retinoblastoma protein (Rb) and p16 were measured by RT-PCR and immunohistochemistry, respectively. Treatment with DHJSD significantly improved the arrangement of collagen fibers in the articular cartilage, as well as its structure and reduced cell degeneration compared with the model group. The mRNA and protein expression levels of cyclin D1, CDK4, CDK6 and Rb in the DHJSD-treated group were significantly increased compared with those in the model group, whereas p16 expression was significantly down-regulated. Taken together, these results indicate that DHJSD treatment promotes chondrocyte proliferation by promoting the G1/S checkpoint transition in the cell cycle and by upregulating the expression of cyclin D1, CDK4, CDK6 and Rb and down-regulating the expression of p16 and this may, in part, explain its clinical efficacy in the treatment of osteoarthritis.

引用

页码：1001 / 1010

页数：10

共 38 条

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β1 integrins regulate chondrocyte rotation, G1 progression, and cytokinesis [J].