Plant products as topical microbicide candidates: assessment of in vitro and in vivo activity against herpes simplex virus type 2

被引:119
作者
Bourne, KZ [1 ]
Bourne, N [1 ]
Reising, SF [1 ]
Stanberry, LR [1 ]
机构
[1] Univ Cincinnati, Coll Med, Dept Pediat, Childrens Hosp Res Fdn,Div Infect Dis, Cincinnati, OH 45229 USA
关键词
herpes simplex virus; genital herpes; topical microbicides; vaginal microbicides; natural products; plant products; sexually transmitted diseases; nonoxynol-9; N-9; carrageenan; cineole; curcumin; eugenol;
D O I
10.1016/S0166-3542(99)00020-0
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
There is considerable interest in developing topical microbicides; products to be used intravaginally by women for protection against sexually transmitted diseases. Many compounds derived from plants have been shown to have antimicrobial properties. We examined 19 such compounds in vitro by plaque reduction assay to determine their activity against a common sexually transmitted pathogen, herpes simplex virus type 2. Compounds with an ED50 less than or equal to 7.0 mg/ml were tested for efficacy in vivo. Four compounds, carrageenan lambda type IV, cineole, curcumin, and eugenol, provided significant protection (P < 0.05) in a mouse model of intravaginal HSV-2 challenge. Eugenol, which provided the greatest protection in mice was also evaluated using the guinea pig model of genital HSV-2 infection where it also demonstrated significant protection. Based on these results, several plant-derived compounds appear to warrant further evaluation as potential microbicides. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:219 / 226
页数:8
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