Sequence variability in viral genome non-coding regions likely contribute to observed differences in viral replication amongst MARV strains

被引：8

作者：

Alonso, Jesus A. ^{[1
,2
]}

Patterson, Jean L. ^{[2
]}

机构：

[1] Univ Texas Hlth Sci Ctr San Antonio, Dept Microbiol & Immunol, San Antonio, TX 78229 USA

[2] Texas Biomed Res Inst, Dept Virol & Immunol, San Antonio, TX 78227 USA

来源：

VIROLOGY | 2013年 / 440卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Marburg virus; Minigenome; Replication; Transcription; VESICULAR STOMATITIS-VIRUS; RESPIRATORY SYNCYTIAL VIRUS; FINGER ANTIVIRAL PROTEIN; NUCLEOPROTEIN-RNA COMPLEX; MARBURG-VIRUS; MESSENGER-RNA; EBOLA-VIRUS; HEMORRHAGIC-FEVER; TRANSCRIPTION; POLYMERASE;

D O I：

10.1016/j.virol.2013.02.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Marburg viruses Musoke (MARV-Mus) and Angola (MARV-Ang) have highly similar genomic sequences. Analysis of viral replication using various assays consistently identified MARV-Ang as the faster replicating virus. Non-coding genomic regions of negative sense RNA viruses are known to play a role in viral gene expression. A comparison of the six non-coding regions using bicistronic minigenomes revealed that the first two non-coding regions (NP/VP35 and VP35/VP40) differed significantly in their transcriptional regulation. Deletion mutation analysis of the MARV-Mus NP/VP35 region further revealed that the MARV polymerase (L) is able to initiate production of the downstream gene without the presence of highly conserved regulatory signals. Bicistronic minigenome assays also identified the VP30 mRNA 5' untranslated region as an rZAP-targeted RNA motif. Overall, our studies indicate that the high variation of MARV non-coding regions may play a significant role in observed differences in transcription and/or replication. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：51 / 63

页数：13

共 35 条

[1] The VSV polymerase can initiate at mRNA start sites located either up or downstream of a transcription termination signal but size of the intervening intergenic region affects efficiency of initiation [J].

Barr, J. N. ;

Tang, Xiaoling ;

Hinman, Edward ;

Shen, Ruizhong ;

Wertz, Gall W. .

VIROLOGY, 2008, 374 (02) :361-370

[2] Role of the intergenic dinucleotide in vesicular stomatitis virus RNA transcription [J].

Barr, JN ;

Whelan, SPJ ;

Wertz, GW .

JOURNAL OF VIROLOGY, 1997, 71 (03) :1794-1801

[3] Transcriptional control of the RNA-dependent RNA polymerase of vesicular stomatitis virus [J].

Barr, JN ;

Whelan, SPJ ;

Wertz, GW .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2002, 1577 (02) :337-353

[4] Structure of N-terminal domain of ZAP indicates how a zinc-finger protein recognizes complex RNA [J].

Chen, Shoudeng ;

Xu, Yihui ;

Zhang, Kuo ;

Wang, Xinlu ;

Sun, Jian ;

Gao, Guangxia ;

Liu, Yingfang .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2012, 19 (04) :430-435

[5] Cross-protection against Marburg virus strains by using a live, attenuated recombinant vaccine [J].

Daddario-DiCaprio, Kathleen M. ;

Geisbert, Thomas W. ;

Geisbert, Joan B. ;

Stroher, Ute ;

Hensley, Lisa E. ;

Grolla, Allen ;

Fritz, Elizabeth A. ;

Feldmann, Friederike ;

Feldmann, Heinz ;

Jones, Steven M. .

JOURNAL OF VIROLOGY, 2006, 80 (19) :9659-9666

[6] Postexposure protection against Marburg haemorrhagic fever with recombinant vesicular stomatitis virus vectors in non-human primates:: an efficacy assessment [J].

Daddario-DiCaprio, KM ;

Geisbert, TW ;

Ströher, U ;

Geisbert, JB ;

Grolla, A ;

Fritz, EA ;

Fernando, L ;

Kagan, E ;

Jahrling, PB ;

Hensley, LE ;

Jones, SM ;

Feldmann, H .

LANCET, 2006, 367 (9520) :1399-1404

[7] Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid protein VP30 [J].

Enterlein, S ;

Volchkov, V ;

Weik, M ;

Kolesnikova, L ;

Volchkova, V ;

Klenk, HD ;

Mühlberger, E .

JOURNAL OF VIROLOGY, 2006, 80 (02) :1038-1043

[8] The Marburg Virus 3′ Noncoding Region Structurally and Functionally Differs from That of Ebola Virus [J].

Enterlein, Sven ;

Schmidt, Kristina M. ;

Schuemann, Michael ;

Conrad, Dominik ;

Kraehling, Verena ;

Olejnik, Judith ;

Muehlberger, Elke .

JOURNAL OF VIROLOGY, 2009, 83 (09) :4508-4519

[9] Mapping the transcription and replication promoters of respiratory syncytial virus [J].

Fearns, R ;

Peeples, ME ;

Collins, PL .

JOURNAL OF VIROLOGY, 2002, 76 (04) :1663-1672

[10] MARBURG VIRUS, A FILOVIRUS - MESSENGER-RNAS, GENE ORDER, AND REGULATORY ELEMENTS OF THE REPLICATION CYCLE [J].

FELDMANN, H ;

MUHLBERGER, E ;

RANDOLF, A ;

WILL, C ;

KILEY, MP ;

SANCHEZ, A ;

KLENK, HD .

VIRUS RESEARCH, 1992, 24 (01) :1-19

← 1 2 3 4 →