Amphipols: Polymers that keep membrane proteins soluble in aqueous solutions

被引：379

作者：

Tribet, C

Audebert, R

Popot, JL

机构：

[1] UNIV PARIS 06,ECOLE SUPER PHYS & CHIM IND,F-75231 PARIS 05,FRANCE

[2] INST BIOL PHYSICOCHIM,CNRS,UNITE PROPRE RECH 9052,F-75005 PARIS 05,FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 26期

关键词：

detergent; bacteriorhodopsin; reaction center; porin; cytochrome b(6)f;

D O I：

10.1073/pnas.93.26.15047

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Amphipols are a new class of surfactants that make it possible to handle membrane proteins in detergent-free aqueous solution as though they were soluble proteins. The strongly hydrophilic backbone of these polymers is grafted with hydrophobic chains, making them amphiphilic. Amphipols are able to stabilize in aqueous solution under their native state four well-characterized integral membrane proteins: (i) bacteriorhodopsin, (ii) a bacterial photosynthetic reaction center, (iii) cytochrome b(6)f, and (iv) matrix porin.

引用

页码：15047 / 15050

页数：4

共 22 条

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