A simple method for constructing E1-and E1/E4-deleted recombinant adenoviral vectors

被引：235

作者：

Mizuguchi, H

Kay, MA

机构：

[1] Stanford Univ, Med Ctr, Sch Med, Dept Pediat, Stanford, CA 94305 USA

[2] Stanford Univ, Med Ctr, Sch Med, Dept Genet, Stanford, CA 94305 USA

[3] Natl Inst Hlth Sci, Div Biol Chem & Biol, Tokyo 1588501, Japan

来源：

HUMAN GENE THERAPY | 1999年 / 10卷 / 12期

关键词：

D O I：

10.1089/10430349950017374

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We previously developed a two-plasmid in vitro ligation method that did not require a recombination step to produce new recombinant E1- or E1/E3-deleted adenoviral vectors. In this study, we have modified the system to improve the simplicity of vector construction and, in addition, to allow for production of an E1/E4-deleted vector.

引用

收藏

页码：2013 / 2017

页数：5

相关论文

共 10 条

[1] AN EFFICIENT AND FLEXIBLE SYSTEM FOR CONSTRUCTION OF ADENOVIRUS VECTORS WITH INSERTIONS OR DELETIONS IN EARLY REGION-1 AND REGION-3 [J].

BETT, AJ ;

HADDARA, W ;

PREVEC, L ;

GRAHAM, FL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (19) :8802-8806

[2] Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli [J].

Chartier, C ;

Degryse, E ;

Gantzer, M ;

Dieterle, A ;

Pavirani, A ;

Mehtali, M .

JOURNAL OF VIROLOGY, 1996, 70 (07) :4805-4810

[3]

Crouzet J, 1997, P NATL ACAD SCI USA, V94, P1414, DOI 10.1073/pnas.94.4.1414

[4] HOMING OF A DNA ENDONUCLEASE GENE BY MEIOTIC GENE CONVERSION IN SACCHAROMYCES-CEREVISIAE [J].

GIMBLE, FS ;

THORNER, J .

NATURE, 1992, 357 (6376) :301-306

[5] A simplified system for generating recombinant adenoviruses [J].

He, TC ;

Zhou, SB ;

da Costa, LT ;

Yu, J ;

Kinzler, KW ;

Vogelstein, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (05) :2509-2514

[6] EFFICIENT MANIPULATION OF THE HUMAN ADENOVIRUS GENOME AS AN INFECTIOUS YEAST ARTIFICIAL CHROMOSOME CLONE [J].

KETNER, G ;

SPENCER, F ;

TUGENDREICH, S ;

CONNELLY, C ;

HIETER, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) :6186-6190

[7] Development of cell lines capable of complementing E1, E4, and protein IX defective adenovirus type 5 mutants [J].

Krougliak, V ;

Graham, FL .

HUMAN GENE THERAPY, 1995, 6 (12) :1575-1586

[8] Recombinant adenoviruses with large deletions generated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo [J].

Lieber, A ;

He, CY ;

Kirillova, I ;

Kay, MA .

JOURNAL OF VIROLOGY, 1996, 70 (12) :8944-8960

[9] CLEAVAGE PATTERN OF THE HOMING ENDONUCLEASE ENCODED BY THE 5TH INTRON IN THE CHLOROPLAST LARGE SUBUNIT RIBOSOMAL-RNA-ENCODING GENE OF CHLAMYDOMONAS-EUGAMETOS [J].

MARSHALL, P ;

LEMIEUX, C .

GENE, 1991, 104 (02) :241-245

[10] Efficient construction of a recombinant adenovirus vector by an improved in vitro ligation method [J].

Mizuguchi, H ;

Kay, MA .

HUMAN GENE THERAPY, 1998, 9 (17) :2577-2583