Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address

被引：45

作者：

Jongsma, MA

Litjens, RHGM

机构：

[1] Plant Res Int, NL-6700 AA Wageningen, Netherlands

[2] Wageningen UR, BioNT, Wageningen, Netherlands

来源：

PROTEOMICS | 2006年 / 6卷 / 09期

关键词：

O-6-alkylguanine-DNA-alkyltransferase; protein array; protein-DNA-conjugate; self-assembly; SNAP-tag (TM);

D O I：

10.1002/pmic.200500654

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The high-throughput deposition of recombinant proteins on chips, beads or biosensor devices would be greatly facilitated by the implementation of self-assembly concepts. DNA-directed immobilization via conjugation of proteins to an oligonucleotide would be preeminently suited for this purpose. Here, we present a unique method to attach a single DNA address to proteins in one step during the purification from the E. coli lysate by fusion to human O-6-alkylguanine-DNA-alkyltransferase (SNAP-tag (TM)) and the Avitag. Use of the conjugates in converting a DNA chip into a protein chip by self assembly is demonstrated.

引用

页码：2650 / 2655

页数：6

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