Human and avian influenza viruses target different cell types in cultures of human airway epithelium

被引:564
作者
Matrosovich, MN [1 ]
Matrosovich, TY
Gray, T
Roberts, NA
Klenk, HD
机构
[1] Univ Marburg, Inst Virol, D-35037 Marburg, Germany
[2] MP Chumakov Inst Poliomyelitis & Viral Encephalit, Moscow 142782, Russia
[3] NIEHS, Mol Carcinogenesis Lab, Res Triangle Pk, NC 27709 USA
[4] Roche Prod Ltd, Welwyn Garden City AL7 3AY, Herts, England
关键词
D O I
10.1073/pnas.0308001101
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The recent human infections caused by H5N1, H9N2, and H7N7 avian influenza viruses highlighted the continuous threat of new pathogenic influenza viruses emerging from a natural reservoir in birds. It is generally believed that replication of avian influenza viruses in humans is restricted by a poor fit of these viruses to cellular receptors and extracellular inhibitors in the human respiratory tract. However, detailed mechanisms of this restriction remain obscure. Here, using cultures of differentiated human airway epithelial cells, we demonstrated that influenza viruses enter the airway epithelium through specific target cells and that there were striking differences in this respect between human and avian viruses. During the course of a single-cycle infection, human viruses preferentially infected nonciliated cells, whereas avian viruses as well as the egg-adapted human virus variant with an avian virus-like receptor specificity mainly infected ciliated cells. This pattern correlated with the predominant localization of receptors for human viruses (2-6-linked sialic acids) on nonciliated cells and of receptors for avian viruses (2-3-linked sialic acids) on ciliated cells. These findings suggest that although avian influenza viruses can infect human airway epithelium, their replication may be limited by a nonoptimal cellular tropism. Our data throw light on the mechanisms of generation of pandemic viruses from their avian progenitors and open avenues for cell level-oriented studies on the replication and pathogenicity of influenza virus in humans.
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页码:4620 / 4624
页数:5
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