Mapping immune reactivity toward Rv2653 and Rv2654:: Two novel low-molecular-mass antigens found specifically in the Mycobacterium tuberculosis complex

被引：53

作者：

Aagaard, C

Brock, I

Olsen, A

Ottenhoff, THM

Weldingh, K

Andersen, P

机构：

[1] Statens Serum Inst, Dept Immunol & Infect Dis, DK-2300 Copenhagen, Denmark

[2] Leiden Univ, Med Ctr, Dept Immunohematol & Blood Transfus, Leiden, Netherlands

来源：

JOURNAL OF INFECTIOUS DISEASES | 2004年 / 189卷 / 05期

关键词：

D O I：

10.1086/381679

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

New tools are urgently needed for the detection of latent tuberculosis (TB). We evaluated the diagnostic potential of 2 novel Mycobacterium tuberculosis complex-specific candidate antigens (Rv2653 and Rv2654) and investigated T cell recognition during natural infection in humans and experimental infection in guinea pigs. Peripheral blood mononuclear cells stimulated with peptide pools covering the full length of Rv2654 induced interferon-gamma release in 10 of 19 patients with TB. Neither Rv2654 single peptides nor Rv2654 pools were recognized by bacille Calmette-Guerin-vaccinated donors. However, peptides from Rv2653 were recognized by both patients group. The cross-reactive epitope(s) in Rv2653 were located in a 36-amino acid stretch in the center of the molecule. Rv2654 also induced M. tuberculosis-specific skin-test responses in 3 of 4 aerosol-infected guinea pigs. Rv2654 is a strongly recognized T cell antigen that is highly specific for TB and has potential as a novel cell-mediated immunity-based TB diagnostic agent.

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页码：812 / 819

页数：8

相关论文

共 39 条

[1] Expression cloning of an immunodominant family of Mycobacterium tuberculosis antigens using human CD4+ T cells [J].

Alderson, MR ;

Bement, T ;

Day, CH ;

Zhu, LQ ;

Molesh, D ;

Skeiky, YAW ;

Coler, R ;

Lewinsohn, DM ;

Reed, SG ;

Dillon, DC .

JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 191 (03) :551-559

[2] Specific immune-based diagnosis of tuberculosis [J].

Andersen, P ;

Munk, ME ;

Pollock, JM ;

Doherty, TM .

LANCET, 2000, 356 (9235) :1099-1104

[3] Antigenic equivalence of human T-cell responses to Mycobacterium tuberculosis-specific RD1-encoded protein antigens ESAT-6 and culture filtrate protein 10 and to mixtures of synthetic peptides [J].

Arend, SM ;

Geluk, A ;

van Meijgaarden, KE ;

van Dissel, JT ;

Theisen, M ;

Andersen, P ;

Ottenhoff, THM .

INFECTION AND IMMUNITY, 2000, 68 (06) :3314-3321

[4] Detection of active tuberculosis infection by T cell responses to early-secreted antigenic target 6-kDa protein and culture filtrate protein 10 [J].

Arend, SM ;

Andersen, P ;

van Meijgaarden, KE ;

Skjot, RLV ;

Subronto, YW ;

van Dissel, JT ;

Ottenhoff, THM .

JOURNAL OF INFECTIOUS DISEASES, 2000, 181 (05) :1850-1854

[5] Comparative genomics of BCG vaccines by whole-genome DNA microarray [J].

Behr, MA ;

Wilson, MA ;

Gill, WP ;

Salamon, H ;

Schoolnik, GK ;

Rane, S ;

Small, PM .

SCIENCE, 1999, 284 (5419) :1520-1523

[6] HETEROGENEITY OF GENOME SIZES AMONG NATURAL ISOLATES OF ESCHERICHIA-COLI [J].

BERGTHORSSON, U ;

OCHMAN, H .

JOURNAL OF BACTERIOLOGY, 1995, 177 (20) :5784-5789

[7] A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10) [J].

Berthet, FX ;

Rasmussen, PB ;

Rosenkrands, I ;

Andersen, P ;

Gicquel, B .

MICROBIOLOGY-UK, 1998, 144 :3195-3203

[8]

Brock I, 2001, INT J TUBERC LUNG D, V5, P462

[9] Bacterial artificial chromosome-based comparative genomic analysis identifies Mycobacterium microti as a natural ESAT-6 deletion mutant [J].

Brodin, P ;

Eiglmeier, K ;

Marmiesse, M ;

Billault, A ;

Garnier, T ;

Niemann, S ;

Cole, ST ;

Brosch, R .

INFECTION AND IMMUNITY, 2002, 70 (10) :5568-5578

[10] A new evolutionary scenario for the Mycobacterium tuberculosis complex [J].

Brosch, R ;

Gordon, SV ;

Marmiesse, M ;

Brodin, P ;

Buchrieser, C ;

Eiglmeier, K ;

Garnier, T ;

Gutierrez, C ;

Hewinson, G ;

Kremer, K ;

Parsons, LM ;

Pym, AS ;

Samper, S ;

van Soolingen, D ;

Cole, ST .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (06) :3684-3689

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