The ribosomal exit tunnel functions as a discriminating gate

被引:414
作者
Nakatogawa, H
Ito, K [1 ]
机构
[1] Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan
[2] Kyoto Univ, Japan Sci & Technol Corp, CREST, Kyoto 6068507, Japan
关键词
D O I
10.1016/S0092-8674(02)00649-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Translation of SecM stalls unless its N-terminal part is "pulled" by the protein export machinery. Here we show that the sequence motif FXXXXWIXXXXGIRAGP that includes a specific arrest point (Pro) causes elongation arrest within the ribosome. Mutations that bypass the elongation arrest were isolated in 23S rRNA and L22 r protein. Such suppressor mutations occurred at a few specific residues of these components, which all face the narrowest constriction of the ribosomal exit tunnel. Thus, we suggest that this region of the exit tunnel interacts with nascent translation products and functions as a discriminating gate.
引用
收藏
页码:629 / 636
页数:8
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