Maintenance of genomic methylation requires a SW12/SNF2-like protein

被引:551
作者
Jeddeloh, JA [1 ]
Stokes, TL [1 ]
Richards, EJ [1 ]
机构
[1] Washington Univ, Dept Biol, St Louis, MO 63130 USA
基金
美国国家科学基金会;
关键词
D O I
10.1038/8803
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Altering cytosine methylation by genetic means leads to a variety of developmental defects in mice(1), plants(2-5) and fungi(6,7) Deregulation of cytosine methylation also has a role in human carcinogenesis(8). In some cases, these defects have been tied to the inheritance of epigenetic alterations (such as chromatin imprints and DNA methylation patterns) that do not involve changes in DNA sequence(3,8-10). Using a forward genetic screen, we identified a gene (DDM1, decrease in DNA methylation) from the flowering plant Arabidopsis thaliana required to maintain normal cytosine methylation patterns(11). Additional ddm1 alleles (som4, 5, 6, 7, 8) were isolated in a selection for mutations that relieved transgene silencing(12) (E.J.R., unpublished data). Loss of DDM1 function causes a 70% reduction of genomic cytosine methylation, with most of the immediate hypomethylation occurring in repeated sequences(11). In contrast, many low-copy sequences initially retain their methylation in ddm1 homozygotes, but lose methylation over time as the mutants are propagated through multiple generations by self-pollination(3,13). The progressive effect of ddm1 mutations on low-copy sequence methylation suggests that ddm1 mutations compromise the efficiency of methylation of newly incorporated cytosines after DNA replication. In parallel with the slow decay of methylation during inbreeding, ddm1 mutants accumulate heritable alterations (mutations or stable epialleles) at dispersed sites in the genome that lead to morphological abnormalities(3,5,14). Here we report that DDM1 encodes a SWI2/SNF2-like protein, implicating chromatin remodelling as an important process for maintenance of DNA methylation and genome integrity.
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页码:94 / 97
页数:4
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