Taxol and 10-deacetylbaccatinIII induce distinct changes in the dynamics of caveolae

被引：5

作者：

Ahmed, Neesar ^{[1
]}

Dasari, Sreekanth ^{[2
]}

Srivastava, Saumya S. ^{[1
]}

Sneh, Amita ^{[1
]}

Ahmad, Absar ^{[2
]}

Khan, M. Islam ^{[2
]}

Krishnasastry, M. V. ^{[1
]}

机构：

[1] Natl Ctr Cell Sci, Pune 411007, Maharashtra, India

[2] Natl Chem Lab, Pune 411007, Maharashtra, India

来源：

FEBS LETTERS | 2008年 / 582卷 / 25-26期

关键词：

Caveolae; Kiss and run dynamic; TIRFM; Taxol; 10-DeacetylbaccatinIII; BaccatinIII; Cytotoxicity;

D O I：

10.1016/j.febslet.2008.09.029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Taxol treatment of HeLa cells resulted in a transient recruitment of Caveolin-1 to the cell surface followed by internalization. Interestingly, 20 min after 10-deacetylbaccatinIII (10-DAB) treatment, the caveolae displayed faster 'kiss and run' dynamics while BaccatinIII (BacIII) did not induce any change. Sustained phosphorylation of Caveolin-1 is observed upon treatment and between Taxol and 10-DAB, the former shows phosphorylated Raf-1, ERK1/2 and hyperphosphorylated Bcl-2 while the later showed much less magnitude of the same. BacIII treatment did not induce phosphorylation of Raf-1 or Bcl-2. It is possible that Taxol might act on multiple targets and the side chain may be crucial. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3595 / 3600

页数：6

共 17 条

[1]

Taxol-induced apoptosis depends on MAP kinase pathways (ERK and p38) and is independent of p53 [J].