Detection of measles virus-induced apoptosis of human monocytic cell line (THP-1) by DNA fragmentation ELISA

被引：18

作者：

Ito, M ^{[1
]}

Yamamoto, T ^{[1
]}

Watanabe, M ^{[1
]}

Ihara, T ^{[1
]}

Kamiya, H ^{[1
]}

Sakurai, M ^{[1
]}

机构：

[1] MIE NATL HOSP,DEPT PEDIAT,TSU,MIE,JAPAN

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 1996年 / 15卷 / 2-3期

关键词：

apoptosis; measles virus; DNA fragmentation; FS-4; human umbilical vein endothelial cells (HUVEC); ICAM-1 (CD54);

D O I：

10.1111/j.1574-695X.1996.tb00061.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

Measles virus (wild strain, Toyoshima strain)-induced cell death is characterized by cell shrinkage, chromatin condensation, and nuclear fragmentation in a human monocytic cell line (THP-I). DNA fragmentation of measles virus-infected THP-1 cells was demonstrated by DNA agarose gel electrophoresis as well as by DNA fragmentation ELISA. When measles virus-infected THP-I cells were cultured on monolayers of fibroblasts or human umbilical vein endothelial cells (HUVEC), the percentage of measles virus antigen-positive THP-1 cells and DNA fragmentation were significantly decreased. Addition of anti-intercellular adhesion molecule (ICAM)-1 (CD54) monoclonal antibody to culture of measles virus-infected THP-I cells reduced significantly DNA fragmentation induced by measles virus. These findings suggest that inhibition of virus spread by fibroblasts and HUVEC reduces apoptosis, and ICAM-1 (CD54) may participate in the DNA fragmentation pathway.

引用

页码：115 / 122

页数：8

共 21 条

[1]

CONSTITUTIVE PRODUCTION OF INFLAMMATORY AND MITOGENIC CYTOKINES BY RHEUMATOID SYNOVIAL FIBROBLASTS [J].