Secretome-based identification of Mac-2 binding protein as a potential oral cancer marker involved in cell growth and motility

被引：45

作者：

Weng, Li-Ping ^{[2
]}

Wu, Chih-Ching ^{[3
]}

Hsu, Bao-Lian ^{[1
]}

Chi, Lang-Ming ^{[3
,6
]}

Liang, Ying ^{[3
]}

Tseng, Ching-Ping ^{[5
]}

Hsieh, Ling-Ling ^{[4
]}

Yu, Jau-Song ^{[2
,3
]}

机构：

[1] Chang Gung Univ, Dept Cell & Mol Biol, Tao Yuan, Taiwan

[2] Chang Gung Univ, Grad Inst Biomed Sci, Tao Yuan, Taiwan

[3] Chang Gung Univ, Proteom Core Lab, Mol Med Res Ctr, Tao Yuan, Taiwan

[4] Chang Gung Univ, Dept Publ Hlth, Tao Yuan, Taiwan

[5] Chang Gung Univ, Grad Inst Med Biotechnol, Tao Yuan, Taiwan

[6] Chang Gung Mem Hosp, Dept Med Res & Dev, Tao Yuan, Taiwan

来源：

JOURNAL OF PROTEOME RESEARCH | 2008年 / 7卷 / 09期

关键词：

oral cancer; biomarker; secretome; Mac-2; BP; growth; migration; invasion;

D O I：

10.1021/pr800042n

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Oral squamous cell carcinoma (OSCC) is the 11th most common cancer worldwide, and is associated with a high death rate. At present, there are no suitable markers for detecting and/or monitoring OSCC in body fluids/tissues. Here, we used 1D SDS-PAGE and MALDI-TOF MS to systematically analyze the secretomes of two OSCC cell lines (OEC-M1 and SCC4). The putative OSCC-related proteins identified in this analysis included the Mac-2 binding protein (Mac-2 BP), which was further found to be overexpressed in OSCC specimens and significantly elevated in the sera of OSCC patients compared to healthy controls. Finally, RNA interference-based knock-down of Mac-2 BP expression in OSCC cells revealed for the first time that Mac-2 BP is involved in regulating growth and motility of OSCC cells.

引用

页码：3765 / 3775

页数：11

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