Endothelin-I-induced oxidative stress in DOCA-salt hypertension involves NADPH-oxidase-independent mechanisms

被引:91
作者
Callera, GE [1 ]
Tostes, RC
Yogi, A
Montezano, ACI
Touyz, RM
机构
[1] Univ Ottawa, Kidney Res Ctr, Ottawa Hlth Res Inst, Ottawa, ON, Canada
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, Brazil
关键词
DOCA (deoxycorticosterone acetate)-salt hypertension; endothelin-1; ETA receptor; NADPH oxidase; oxidative stress;
D O I
10.1042/CS20050307
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
We have demonstrated recently [Callera, Touyz, Teixeira, Muscara, Carvalho, Fortes, Schiffrin and Tostes (2003) Hypertension 42, 811-817] that increased vascular oxidative stress in DOCA (deoxycorticosterone acetate)-salt rats is associated with activation of the ET (endothelin) system via ETA receptors. The exact source of ET-I-mediated oxidative stress remains unclear. The aim of the present study was to investigate whether ET-I increases generation of ROS (reactive oxygen species) in DOCA-salt hypertension through NAD PH-oxidase-depen dent mechanisms. Xanthine oxidase, eNOS (endothelial nitric oxide synthase) and COX-2 (cyclo-oxygenase-2) were also examined as potential ET-I sources of ROS as well as mitochondrial respiration. DOCA-salt and control UniNX (uninephrectomized) rats were treated with the ETA antagonist BMS 182874 (40 mg (.) day(-1) (.) kg(-1) of body weight) or vehicle. Plasma TBARS (thiobarbituric acid-reacting substances) were increased in DOCA-salt compared with UniNX rats. Activity of NADPH and xanthine oxidases in aorta, mesenteric arteries and heart was increased in DOCA-salt rats. BMS 182874 decreased plasma TBARS levels without influencing NADPH and xanthine oxidase activities in DOCA-salt rats. Increased p22(phox) protein expression and increased p47(phox) membrane translocation in arteries from DOCA-salt by rats were not affected by BMS 182874 treatment. Increased eNOS and COX-2 expression, also observed in aortas from DOCA-salt rats, was unaltered by BMS182874. Increased mitochondrial generation of ROS in DOCA-salt rats was normalized by BMS182874. ETA antagonism also increased the expression of mitochondrial MnSOD (manganese superoxide dismutase) in DOCA-salt rats. In conclusion, activation of NADPH oxidase does not seem to be the major source of oxidative stress induced by ET-I/ ETA in DOCA-salt hypertension, which also appears to be independent of increased activation of xanthine oxidase or eNOS/COX-2 overexpression. Mitochondria. may play a role in ET-I-driven oxidative stress, as evidenced by increased mitochondrial-derived ROS in this model of hypertension.
引用
收藏
页码:243 / 253
页数:11
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