A highly purified RNA polymerase II elongation control system

被引:152
作者
Renner, DB
Yamaguchi, Y
Wada, T
Handa, H
Price, DH [1 ]
机构
[1] Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA
[2] Tokyo Inst Technol, Fac Biosci & Biotechnol, Yokohama, Kanagawa 2268501, Japan
[3] Tokyo Inst Technol, Frontier Collaborat Res Ctr, Yokohama, Kanagawa 2268501, Japan
关键词
D O I
10.1074/jbc.M104967200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Studying the sensitivity of transcription to the nucleotide analog 5,6-dichloro-1-beta -D-ribofuranosylbenzimidazole has led to the discovery of a number of proteins involved in the regulation of transcription elongation by RNA polymerase II. We have developed a highly purified elongation control system composed of three purified proteins added back to isolated RNA polymerase II elongation complexes. Two of the proteins, 5,6-dichloro-1-13D-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF), act as negative transcription elongation factors by increasing the time the polymerase spent at pause sites. reverses the negative effect of DSIF and NELF through a mechanism dependent on its kinase activity. TFIIF is a general initiation factor that positively affects elongation by decreasing pausing. We show that TFIIF functionally competes with DSIF and NELF, and this competition is dependent on the relative concentrations of TFIIF and NELF.
引用
收藏
页码:42601 / 42609
页数:9
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