Hydrogen peroxide accumulation in Medicago truncatula roots colonized by the arbuscular mycorrhiza-forming fungus Glomus intraradices

被引:151
作者
Salzer, P [1 ]
Corbière, H [1 ]
Boller, T [1 ]
机构
[1] Univ Basel, Inst Bot, CH-4056 Basel, Switzerland
关键词
arbuscular mycorrhiza; Glomus; H2O2; synthesis; Medicago; plant defence; symbiosis;
D O I
10.1007/s004250050565
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The diaminobenzidine (DAB) staining technique was used to examine the accumulation of H2O2 in parts of roots of Medicago truncatula Gaertn. colonized by the arbuscular mycorrhiza (AM)-forming fungus Glomus intraradices Schenk and Smith. At the cellular level, the combination of bright-field and fluorescence microscopy revealed that a brownish stain, indicative of H2O2 accumulation was present within cortical root cells in the space occupied by arbuscules. Accumulation of H2O2 was especially pronounced in cells containing arbuscules that were clumped and less branched. Moreover, H2O2 accumulated around hyphal tips attempting to penetrate a host cell. In contrast, no H2O2 accumulation was observed in hyphal tips growing along the middle lamella, or in appressoria or vesicles. On the basis of these findings we suggest that a locally restricted oxidative burst is involved in the temporal and spatial control of the intracellular colonization of M. truncatula cells by the AM-forming fungus G. intraradices.
引用
收藏
页码:319 / 325
页数:7
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