Super-high-speed liquid chromatography of proteins and peptides on non-porous Micra NPS-RP packings

被引：45

作者：

Issaeva, T ^{[1
]}

Kourganov, A ^{[1
]}

Unger, K ^{[1
]}

机构：

[1] Univ Mainz, Inst Anorgan Chem & Analyt Chem, D-55099 Mainz, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 846卷 / 1-2期

关键词：

stationary phases; LC; proteins; peptides;

D O I：

10.1016/S0021-9673(99)00360-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The new generation of non-porous silica RP packings commercially available from Micra Scientific was tested for separations of peptides and proteins by means of the gradient HPLC. Extremely high-speed separations were achieved using conventional chromatographic equipment: six proteins could be completely separated within six seconds. Tryptic digest peptides could be resolved in more then 40 components within 2-3 min. The effect of the experimental parameters such as temperature, flow rate etc. was investigated. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：13 / 23

页数：11

共 20 条

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