The N-end rule pathway promotes seed germination and establishment through removal of ABA sensitivity in Arabidopsis

被引:152
作者
Holman, Tara J. [1 ,2 ]
Jones, Peter D. [1 ]
Russell, Laurel [4 ]
Medhurst, Anne [1 ]
Tomas, Susana Ubeda [3 ]
Talloji, Prabhavathi [5 ,6 ]
Marquez, Julietta [1 ]
Schmuths, Heike [1 ]
Tung, Swee-Ang [1 ]
Taylor, Ian [1 ]
Footitt, Steven [2 ]
Bachmair, Andreas [5 ,6 ]
Theodoulou, Frederica L. [2 ]
Holdsworth, Michael J. [1 ,3 ]
机构
[1] Univ Nottingham, Dept Plant & Crop Sci, Sch BioSci, Nottingham LE12 5RD, England
[2] Rothamsted Res, Crop Performance & Improvement Div, Harpenden AL5 2JQ, Herts, England
[3] Univ Nottingham, Ctr Plant Integrat Biol, Sch BioSci, Nottingham LE12 5RD, England
[4] Univ Bristol, Long Ashton Res Stn, Dept Agr Sci, Bristol BS41 9AF, Avon, England
[5] Max Planck Inst Plant Breeding Res, Dept Plant Dev Biol, D-50829 Cologne, Germany
[6] Univ Vienna, Dept Biochem, Max F Perutz Labs, A-1030 Vienna, Austria
基金
英国生物技术与生命科学研究理事会;
关键词
abscisic acid; aminoacyl tRNA protein transferase; lipid bodies; targeted protein degradation; ABSCISIC-ACID; BETA-OXIDATION; GIBBERELLIN; ENCODES; PROTEIN; DORMANCY; MUTANTS; LIGASE; GENE; COMATOSE;
D O I
10.1073/pnas.0810280106
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The N-end rule pathway targets protein degradation through the identity of the amino-terminal residue of specific protein substrates. Two components of this pathway in Arabidopsis thaliana, PROTEOLYSIS6 (PRT6) and arginyl-tRNA: protein arginyltransferase ( ATE), were shown to regulate seed after-ripening, seedling sugar sensitivity, seedling lipid breakdown, and abscisic acid (ABA) sensitivity of germination. Sensitivity of prt6 mutant seeds to ABA inhibition of endosperm rupture reduced with after-ripening time, suggesting that seeds display a previously undescribed window of sensitivity to ABA. Reduced root growth of prt6 alleles and the ate1 ate2 double mutant was rescued by exogenous sucrose, and the breakdown of lipid bodies and seed-derived triacylglycerol was impaired in mutant seedlings, implicating the N-end rule pathway in control of seed oil mobilization. Epistasis analysis indicated that PRT6 control of germination and establishment, as exemplified by ABA and sugar sensitivity, as well as storage oil mobilization, occurs at least in part via transcription factors ABI3 and ABI5. The N-end rule pathway of protein turnover is therefore postulated to inactivate as-yet unidentified key component(s) of ABA signaling to influence the seed-to-seedling transition.
引用
收藏
页码:4549 / 4554
页数:6
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