Antisense inhibition of GAS, the human homologue of the yeast chromosome segregation gene CSE1, interferes with mitosis in HeLa cells

被引：26

作者：

Ogryzko, VV

Brinkmann, E

Howard, BH

Pastan, I

Brinkmann, U

机构：

[1] NICHHD,LAB MOL GROWTH REGULAT,NIH,BETHESDA,MD 20892

[2] NCI,MOL BIOL LAB,DIV BASIC SCI,NIH,BETHESDA,MD 20892

来源：

BIOCHEMISTRY | 1997年 / 36卷 / 31期

关键词：

D O I：

10.1021/bi970236o

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have analyzed the effects on HeLa cells of reduction of the CAS protein, the human homologue to yeast chromosome segregation protein CSE1. Expression of CAS antisense cDNA decreases the amount of CAS protein in HeLa cells and perturbs progression from G2 (retards transition from G2) to G1 in the cell cycle. Increased levels of cyclin B in CAS antisense transfected cells correlated with an arrest in G2 phase or mitosis. This arrest upon CAS attenuation is consistent with observations that yeast with CSE1 mutations are defective in mitosis and cyclin B degradation.

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页码：9493 / 9500

页数：8

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