Quantitative phase contrast microscopy of living cells by numerical reconstruction of digital holograms

被引：6

作者：

Cuche, E ^{[1
]}

Marquet, P ^{[1
]}

Magistretti, PJ ^{[1
]}

Depeursinge, C ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Opt Appl, CH-1015 Lausanne, Switzerland

来源：

OPTICAL DIAGNOSTICS AND LIVING CELLS II, PROCEEDINGS OF | 1999年 / 3604卷

关键词：

holography; CCD camera; phase contrast microscopy; astrocytes; cellular microscopy;

D O I：

10.1117/12.349218

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We describe a new method of optical microscopy which allows simultaneously intensity and quantitative phase contrast imaging. The method is based on a new holographic imaging technique which involves a CCD camera as recording device and a numerical method for the reconstruction. An application to phase contrast imaging of living astrocytes is presented.

引用

页码：84 / 89

页数：6

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