Present status of the application of cryopreserved hepatocytes in the evaluation of xenobiotics: consensus of an international expert panel

被引:101
作者
Li, AP
Gorycki, PD
Hengstler, JG
Kedderis, GL
Koebe, HG
Rahmani, R
de Sousas, G
Silva, JM
Skett, P
机构
[1] In Vitro Technol, Baltimore, MD 21227 USA
[2] SmithKline Beecham Pharmaceut, Dept Drug Metab & Pharmacokinet, King Of Prussia, PA 19406 USA
[3] Chem Ind Inst Toxicol, Res Triangle Pk, NC 27709 USA
[4] Johannes Gutenberg Univ Mainz, Inst Toxicol, D-55131 Mainz, Germany
[5] Univ Munich, Klinikum Grosshadern, Dept Surg, D-81377 Munich, Germany
[6] Ctr Rech Agron, INSEAM, F-06606 Antibes, France
[7] Merck Frosst Canada Inc, Med Chem, Pointe Claire, PQ H9R 4P8, Canada
[8] Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland
关键词
cryopreserved hepatocytes; xenobiotics; isolation;
D O I
10.1016/S0009-2797(99)00081-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Successful cryopreservation of freshly isolated hepatocytes would significantly decrease the need for freshly-procured livers for the preparation of hepatocytes for experimentation. Hepatocytes can be prepared, cryopreserved, and used for experimentation as needed at different times after isolation. Cryopreservation is especially important for research with human hepatocytes because of the limited availability of fresh human livers. Based on the cumulative experience of this international expert panel, a consensus was reached on the various aspects of hepatocyte cryopreservation, including cryopreservation and thawing procedures and applications of the cryopreserved hepatocytes. Key to successful cryopreservation includes slow addition of cryopreservants, controlled-rate freezing with adjustment for the heat of crystallization, storage at - 150 degrees C, and rapid thawing. There is a general consensus that cryopreserved hepatocytes are useful for short-term xenobiotic metabolism and cytotoxicity evaluation. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
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页码:117 / 123
页数:7
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