Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy

被引：382

作者：

Mansouri, S ^{[1
]}

Lavigne, P ^{[1
]}

Corsi, K ^{[1
]}

Benderdour, M ^{[1
]}

Beaumont, E ^{[1
]}

Fernandes, JC ^{[1
]}

机构：

[1] Hop Sacre Coeur, Ctr Rech, Lab Rech Orthopedie, Montreal, PQ H4J 1C5, Canada

来源：

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS | 2004年 / 57卷 / 01期

基金：

加拿大健康研究院;

关键词：

non-viral vectors; chitosan; nanoparticles; transfection efficiency; gene therapy;

D O I：

10.1016/S0939-6411(03)00155-3

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Currently, the major drawback of gene therapy is the gene transfection rate. The two main types of vectors that are used in gene therapy are based on viral or non-viral gene delivery systems. The viral gene delivery system shows a high transfection yield but it has many disadvantages, such as oncogenic effects and immunogenicity. However, cationic polymers, like chitosan, have potential for DNA complexation and may be useful as non-viral vectors for gene therapy applications. Chitosan is a natural non-toxic polysaccharide, it is biodegradable and biocompatible, and protects DNA against DNase degradation and leads to its condensation. The objective of this paper was to summarize the state of the art in gene therapy and particularly the use of chitosan to improve the transfection efficiency in vivo and in vitro. (C) 2003 Published by Elsevier B.V.

引用

页码：1 / 8

页数：8

共 61 条

[1]

ALAN DO, 2002, J AM CHEM SOC, V124, P8

[2]

AUDOUY SA, 2002, PHARM RES, P1599

[3] pH-sensitive cationic polymer gene delivery vehicle:: N-Ac-poly(L-histidine)-graft-poly(L-lysine) comb shaped polymer [J].

Benns, JM ;

Choi, JS ;

Mahato, RI ;

Park, JS ;

Kim, SW .

BIOCONJUGATE CHEMISTRY, 2000, 11 (05) :637-645

[4] Application of membrane-based dendrimer/DNA complexes for solid phase transfection in vitro and in vivo [J].

Bielinska, AU ;

Yen, A ;

Wu, HL ;

Zahos, KM ;

Sun, R ;

Weiner, ND ;

Baker, JR ;

Roessler, BJ .

BIOMATERIALS, 2000, 21 (09) :877-887

[5] GENE-THERAPY IN PERIPHERAL-BLOOD LYMPHOCYTES AND BONE-MARROW FOR ADA(-) IMMUNODEFICIENT PATIENTS [J].

BORDIGNON, C ;

NOTARANGELO, LD ;

NOBILI, N ;

FERRARI, G ;

CASORATI, G ;

PANINA, P ;

MAZZOLARI, E ;

MAGGIONI, D ;

ROSSI, C ;

SERVIDA, P ;

UGAZIO, AG ;

MAVILIO, F .

SCIENCE, 1995, 270 (5235) :470-475

[6] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE [J].

BOUSSIF, O ;

LEZOUALCH, F ;

ZANTA, MA ;

MERGNY, MD ;

SCHERMAN, D ;

DEMENEIX, B ;

BEHR, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7297-7301

[7] Gene delivery with synthetic (non viral) carriers [J].

Brown, MD ;

Schätzlein, AG ;

Uchegbu, IF .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2001, 229 (1-2) :1-21

[8] Direct cutaneous gene delivery in a human genetic skin disease [J].

Choate, KA ;

Khavari, PA .

HUMAN GENE THERAPY, 1997, 8 (14) :1659-1665

[9] Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles [J].

Corsi, K ;

Chellat, F ;

Yahia, L ;

Fernandes, JC .

BIOMATERIALS, 2003, 24 (07) :1255-1264

[10] Chitosan-based nanoparticles for topical genetic immunization [J].

Cui, ZR ;

Mumper, RJ .

JOURNAL OF CONTROLLED RELEASE, 2001, 75 (03) :409-419

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