Molecular cloning and characterization of Toll-like receptors 1-10 in sheep

被引：54

作者：

Chang, Jung-Su ^{[2
]}

Russell, George C. ^{[2
]}

Jann, Oliver ^{[3
]}

Glass, Elizabeth J. ^{[3
,4
]}

Werling, Dirk ^{[4
]}

Haig, David M. ^{[1
]}

机构：

[1] Univ Nottingham, Sch Vet Med & Sci, Loughborough LE12 5RD, England

[2] Moredun Res Inst, Penicuik EH26 0PZ, Midlothian, Scotland

[3] Univ Edinburgh, Roslin Inst, Roslin EH25 9PS, Midlothian, Scotland

[4] Univ London Royal Vet Coll, Hatfield AL9 7TA, Herts, England

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2009年 / 127卷 / 1-2期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Ovine; Innate immunity; Toll-like receptors; Phylogeny; PATTERN-RECOGNITION RECEPTORS; LEUCINE-RICH REPEATS; BOVINE; GENE; EXPRESSION; PROTEIN; TLR1;

D O I：

10.1016/j.vetimm.2008.09.026

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Toll-like receptors (TLRs) are pattern-recognition receptors that trigger innate immune responses and stimulate adaptive immunity. Currently, only partial information is available for sheep TLR genes. The aims of this study were to clone and sequence the coding regions of all 10 ovine TLR genes and compare the sequences with those of other mammalian species. The coding sequences for ovine TLRs 1-10 and the 3'-untranslated sequences for ovine TLR1, 6 and 10 have been obtained. Ovine TLR6 exhibited a distinctive 3'-end sequence that resembled a rare splice variant of bovine TLR6, but appeared to represent the major TLR6 transcript in the sheep. qRT-PCR confirmed the presence of TLR transcripts in blood mononuclear cells, alveolar macrophages, keratinocytes and lymph node tissues. Comparative sequence analysis showed that the sheep TLRs share high sequence similarity with the respective cattle, pig, human and mouse genes and are likely derived from the same ancestral sequence. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：94 / 105

页数：12

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