Fed-batch culture of Sf-9 cells supports 3x10(7) cells per ml and improves baculovirus-expressed recombinant protein yields

被引：31

作者：

Bedard, C ^{[1
]}

Perret, S ^{[1
]}

Kamen, AA ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,BIOTECHNOL RES INST,LARGE SCALE BIOPROC GRP,MONTREAL,PQ H4P 2R2,CANADA

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 07期

关键词：

D O I：

10.1023/A:1018378529299

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Yields of cell and baculovirus-expressed recombinant protein in Sf-9 cultures were improved using fed-batch addition of glucose, amino acids, lipids, yeastolate ultrafiltrate, vitamins, iron and trace metals. Using this approach, the highest maximum cell and recombinant p-galactosidase volumetric yields obtained, 3 x 10(7).ml(-1) and 900 units.ml(-1), respectively, were both approximately triple their respective values in unamended cultures.

引用

页码：629 / 632

页数：4

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