Cytotoxic T-cell recognition of HIV-1 cross-clade and clade-specific epitopes in HIV-1-infected Thai and Japanese patients

被引:32
作者
Fukada, K
Tomiyama, H
Wasi, C
Matsuda, T
Kusagawa, S
Sato, H
Oka, S
Takebe, Y
Takiguchi, M
机构
[1] Kumamoto Univ, Ctr AIDS Res, Div Viral Immunol, Kumamoto 8600811, Japan
[2] Chemo Sero Therapeut Res Inst, Kumamoto, Japan
[3] Mahidol Univ, Siriraj Hosp, Fac Med, Dept Microbiol, Bangkok 10700, Thailand
[4] Natl Inst Infect Dis, Tokyo, Japan
[5] Int Med Ctr Japan, AIDS Clin Ctr, Tokyo, Japan
关键词
HIV-1 clade E; non-clade B; cross-clade; CTL epitope; Asia; HLA-A*1101;
D O I
10.1097/00002030-200203290-00005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Objective: To identify and characterize cytotoxic T-cell (CTL) epitopes for HIV-1 clade E using eight known HLA-A*1101-restricted HIV-1 clade B epitopes. Methods: Induction of clade E-specific CTL was examined by stimulating peripheral blood mononuclear cells (PBMC) from clade E-infected Thai individuals with the clade E-specific peptide corresponding to the clade B epitopes. Cross-clade and clade-specific CTL recognition for these epitopes was analysed using CTL clones and bulk CTL specific for these epitopes. To clarify the presentation of these epitopes in HIV-1-infected T cells, CTL recognition for the clade E-specific and cross-clade epitopes was investigated using CD4CXCR4 cells infected with an HIV-1 clade E clone. Results: Three epitopes, which are identical among clades A-E, were recognized as cross-clade CTL epitopes in both individuals. Clade B and E sequences corresponding to three epitopes were recognized as clade-specific epitopes in clade B-infected and clade E-infected individuals, respectively. In contrast, clade E-specific peptides corresponding to two other clade B epitopes failed to elicit clade E-specific CTL. CTL specific for the three cross-clade and three clade E-specific epitopes effectively lysed target cells infected with HIV-1 clade E virus. Conclusions: These six epitopes are found to be processed naturally in HIV-1 clade E-infected cells. We show here that a strategy utilizing HIV-1 clade B epitopes is very useful for identifying clade E CTL epitopes. (C) 2002 Lippincott Williams Wilkins.
引用
收藏
页码:701 / 711
页数:11
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