Biological characteristics and propagation of human rhinovirus-C in differentiated sinus epithelial cells

被引：47

作者：

Ashraf, Shamaila ^{[1
]}

Brockman-Schneider, Rebecca ^{[1
]}

Bochkov, Yury A. ^{[1
]}

Pasic, Thomas R. ^{[2
]}

Gern, James E. ^{[1
,3
]}

机构：

[1] Univ Wisconsin, Sch Med & Publ Hlth, Dept Pediat, Madison, WI 53792 USA

[2] Univ Wisconsin, Sch Med & Publ Hlth, Dept Surg, Madison, WI 53792 USA

[3] Univ Wisconsin, Sch Med & Publ Hlth, Dept Med, Madison, WI 53792 USA

来源：

VIROLOGY | 2013年 / 436卷 / 01期

关键词：

Human rhinovirus-C; Air-liquid interface; Differentiated sinus epithelium; qRT-PCR; Temperature sensitivity; pH sensitivity; INFECTIONS; SEQUENCE; ASTHMA; EXACERBATIONS; SYSTEM;

D O I：

10.1016/j.virol.2012.11.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Information about the basic biological properties of human rhinovirus-C (HRV-C) viruses is lacking due to difficulties with culturing these viruses. Our objective was to develop a cell culture system to grow HRV-C. Epithelial cells from human sinuses (HSEC) were differentiated at air-liquid interface (ALI). Differentiated cultures supported 1-2 logs growth of HRV-C15 as detected by quantitative RT-PCR. Two distinguishing features of HRVs are acid lability and optimal growth at 33-34 degrees C. We used this system to show that HRV-C15 is neutralized by low pH (4.5). In contrast to most HRV types, replication of HRV-C15 and HRV-C41 was similar at 34 and 37 degrees C. The HSEC ALI provides a useful tool for quantitative studies of HRV-C replication. The ability of HRV-C to grow equally well at 34 degrees C and 37 degrees C may contribute to the propensity for HRV-C to cause lower airway illnesses in infants and children with asthma. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：143 / 149

页数：7

共 35 条

[1] 2 GROUPS OF RHINOVIRUSES REVEALED BY A PANEL OF ANTIVIRAL COMPOUNDS PRESENT SEQUENCE DIVERGENCE AND DIFFERENTIAL PATHOGENICITY [J].

ANDRIES, K ;

DEWINDT, B ;

SNOEKS, J ;

WOUTERS, L ;

MOEREELS, H ;

LEWI, PJ ;

JANSSEN, PAJ .

JOURNAL OF VIROLOGY, 1990, 64 (03) :1117-1123

[2] Molecular characterization and distinguishing features of a novel human rhinovirus (HRV) C, HRVC-QCE, detected in children with fever, cough and wheeze during 2003 [J].

Arden, K. E. ;

Faux, C. E. ;

O'Neill, N. T. ;

McErlean, P. ;

Nitsche, A. ;

Lambert, S. B. ;

Nissen, M. D. ;

Sloots, T. P. ;

Mackay, I. M. .

JOURNAL OF CLINICAL VIROLOGY, 2010, 47 (03) :219-223

[3] Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections [J].

Arden, Katherine E. ;

McErlean, Peter ;

Nissen, Michael D. ;

Sloots, Theo P. ;

Mackay, Ian M. .

JOURNAL OF MEDICAL VIROLOGY, 2006, 78 (09) :1232-1240

[4] Newly identified human rhinoviruses: molecular methods heat up the cold viruses [J].

Arden, Katherine E. ;

Mackay, Ian M. .

REVIEWS IN MEDICAL VIROLOGY, 2010, 20 (03) :156-176

[5] Association between human rhinovirus C and severity of acute asthma in children [J].

Bizzintino, J. ;

Lee, W-M. ;

Laing, I. A. ;

Vang, F. ;

Pappas, T. ;

Zhang, G. ;

Martin, A. C. ;

Khoo, S-K. ;

Cox, D. W. ;

Geelhoed, G. C. ;

McMinne, P. C. ;

Goldblatt, J. ;

Gern, J. E. ;

Le Souef, P. N. .

EUROPEAN RESPIRATORY JOURNAL, 2011, 37 (05) :1037-1042

[6] Molecular modeling, organ culture and reverse genetics for a newly identified human rhinovirus C [J].

Bochkov, Yury A. ;

Palmenberg, Ann C. ;

Lee, Wai-Ming ;

Rathe, Jennifer A. ;

Amineva, Svetlana P. ;

Sun, Xin ;

Pasic, Thomas R. ;

Jarjour, Nizar N. ;

Liggett, Stephen B. ;

Gern, James E. .

NATURE MEDICINE, 2011, 17 (05) :627-U152

[7] Global distribution of novel rhinovirus genotype [J].

Briese, Thomas ;

Renwick, Neil ;

Venter, Marietjie ;

Jarman, Richard G. ;

Ghosh, Dhrubaa ;

Koendgen, Sophie ;

Shrestha, Sanjaya K. ;

Hoegh, A. Mette ;

Casas, Inmaculada ;

Adjogoua, Edgard Valerie ;

Akoua-Koffi, Chantal ;

Myint, Khin Saw ;

Williams, David T. ;

Chidlow, Glenys ;

van den Berg, Ria ;

Calvo, Cristina ;

Koch, Orienka ;

Palacios, Gustavo ;

Kapoor, Vishal ;

Villari, Joseph ;

Dominguez, Samuel R. ;

Holmes, Kathryn V. ;

Harnett, Gerry ;

Smith, David ;

Mackenzie, John S. ;

Ellerbrok, Heinz ;

Schweiger, Brunhilde ;

Schonning, Kristian ;

Chadha, Mandeep S. ;

Leendertz, Fabian H. ;

Mishra, A. C. ;

Gibbons, Robert V. ;

Holmes, Edward C. ;

Lipkin, W. Ian .

EMERGING INFECTIOUS DISEASES, 2008, 14 (06) :944-947

[8]

DIMMOCK NJ, 1964, BRIT J EXP PATHOL, V45, P271

[9] Human Rhinovirus Infections in Rural Thailand: Epidemiological Evidence for Rhinovirus as Both Pathogen and Bystander [J].

Fry, Alicia M. ;

Lu, Xiaoyan ;

Olsen, Sonja J. ;

Chittaganpitch, Malinee ;

Sawatwong, Pongpun ;

Chantra, Somrak ;

Baggett, Henry C. ;

Erdman, Dean .

PLOS ONE, 2011, 6 (03)

[10] ACID-INDUCED STRUCTURAL-CHANGES IN HUMAN RHINOVIRUS-14 - POSSIBLE ROLE IN UNCOATING [J].

GIRANDA, VL ;

HEINZ, BA ;

OLIVEIRA, MA ;

MINOR, I ;

KIM, KH ;

KOLATKAR, PR ;

ROSSMANN, MG ;

RUECKERT, RR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10213-10217

← 1 2 3 4 →