The methyl-CpG binding transcriptional repressor MeCP2 stably associates with nucleosomal DNA

被引：148

作者：

Chandler, SP ^{[1
]}

Guschin, D ^{[1
]}

Landsberger, N ^{[1
]}

Wolffe, AP ^{[1
]}

机构：

[1] NICHHD, Mol Embryol Lab, NIH, Bethesda, MD 20892 USA

来源：

BIOCHEMISTRY | 1999年 / 38卷 / 22期

关键词：

D O I：

10.1021/bi990224y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the interactions of the methyl-CpG binding transcriptional repressor MeCP2 with nucleosomal DNA. We find that MeCP2 forms discrete complexes with nucleosomal DNA associating with methyl-CpGs exposed in the major groove via the methyl-CDG-binding domain (MBD). In addition to the MBD, the carboxyl-terminal segment of MeCP2 facilitates binding both to naked DNA and to the nucleosome core. These observations provide a molecular mechanism by which MeCP2 can gain access to chromatin in order to target corepressor complexes that further modify chromatin structure.

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页码：7008 / 7018

页数：11

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