The potential for reintroduction of tumor cells during intraoperative blood salvage: Reduction of risk with use of the RC-400 leukocyte depletion filter

被引:70
作者
Edelman, MJ
Potter, P
Mahaffey, KG
Frink, R
Leidich, RB
机构
[1] UNIV CALIF DAVIS,VET AFFAIRS NO CALIF HLTH CARE SYST,DIV HEMATOL ONCOL,DAVIS,CA 95616
[2] USN,MED CTR,DEPT ANESTHESIA,SAN DIEGO,CA 92152
[3] USN HOSP,DEPT UROL,AGANA,GU
[4] USN,MED CTR,DEPT UROL,OAKLAND,CA
[5] TEXAS TECH UNIV,DEPT UROL,LUBBOCK,TX 79409
关键词
D O I
10.1016/S0090-4295(99)80411-7
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Objectives. Intraoperative autotransfusion of shed blood is widely utilized in surgery. However, several studies have raised concern about the transmission of tumor cells during oncologic procedures. We compared the ability of a leukocyte depletion filter (RC-400; LDF) to a standard red blood cell filter (SBF) to remove tumor cells derived from urologic malignancies. Methods. Cells were suspended in media and passed through a SBF or a LDF. The filtrate was evaluated for the presence of viable cells utilizing the trypan blue exclusion method as well as cell culture. in a second experiment, cells were suspended in fresh bovine blood and processed through a cell saver apparatus followed by filtration with either a SBF or a LDF, Aliquots were cultured after admixture with blood, after processing, and after filtration. Results. The LDF was able to remove tumor cells completely, as demonstrated by both counting with the trypan blue exclusion test and by cell culture. In contrast, admixture with blood, processing through the cell saver apparatus nor a standard red blood cell filter removed these cells. Conclusions. Tumor cells derived from urologic malignancies are easily removed with a LDF but not with a SBF. Filtration of blood salvaged at the time of uro-oncologic surgery with a LDF but not with a SBF reduces the potential for reinfusion of viable tumor cells.
引用
收藏
页码:179 / 181
页数:3
相关论文
共 14 条
[1]   SEPARATION OF MALIGNANT-CELLS DURING AUTO-TRANSFUSION [J].
DALE, RF ;
KIPLING, RM ;
SMITH, MF ;
COLLIER, DS ;
SMITH, PJ .
BRITISH JOURNAL OF SURGERY, 1988, 75 (06) :581-581
[2]   PERIOPERATIVE INTERVENTIONS TO DECREASE TRANSFUSION OF ALLOGENEIC BLOOD PRODUCTS [J].
ERETH, MH ;
OLIVER, WC ;
SANTRACH, PJ .
MAYO CLINIC PROCEEDINGS, 1994, 69 (06) :575-586
[3]  
GIORDANO GF, 1993, MANAGEMENT SURG BLOO
[4]  
HANSEN E, 1995, ARCH SURG-CHICAGO, V130, P387
[5]  
HANSEN E, 1993, ANESTHESIOLOGY, V79, pA3
[6]  
HART OJ, 1989, SURG GYNECOL OBSTET, V168, P302
[7]  
Homann B, 1984, Acta Anaesthesiol Belg, V35 Suppl, P51
[8]   INTRAOPERATIVE AUTOTRANSFUSION IN UROLOGIC ONCOLOGY [J].
KLIMBERG, I ;
SIROIS, R ;
WAJSMAN, Z ;
BAKER, J .
ARCHIVES OF SURGERY, 1986, 121 (11) :1326-1329
[9]  
LEIDICH RB, 1996, IN PRESS INFECT UROL
[10]   AUTOLOGOUS TRANSFUSION - AN ALTERNATIVE TO TRANSFUSION WITH BANKED BLOOD DURING SURGERY FOR CANCER [J].
MILLER, GV ;
RAMSDEN, CW ;
PRIMROSE, JN .
BRITISH JOURNAL OF SURGERY, 1991, 78 (06) :713-715