Identification of ether a go-go and calcium-activated potassium channels in human melanoma cells

被引:87
作者
Meyer, R
Schönherr, R
Gavrilova-Ruch, O
Wohlrab, W
Heinemann, SH
机构
[1] Klinikum Univ Jena, Arbeitsgrp Mol & Zellulare Biophys, D-07747 Jena, Germany
[2] Univ Halle Wittenberg, Fak Med, Dermatol Klin, D-06097 Halle, Germany
关键词
potassium channel; melanoma; ether a go-go channel; intracellular Ca2+; proliferation; patch clamp;
D O I
10.1007/s002329900563
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ion channels and intracellular Ca2+ are thought to be involved in cell proliferation and may play a role in tumor development. We therefore characterized Ca2+ regulated potassium channels in the human melanoma cell lines IGR1, IPC298, and IGR39 using electrophysiological and molecular biological methods. All cell lines expressed outwardly rectifying K+ channels. Rapidly activating delayed rectifier channels were detected in IGR39 cells. The activation kinetics of voltage-gated K+ channels in IRG1 and IPC298 cells displayed characteristics of ether a go-go (eag) channels as they were much slower and depended both on the holding potential and on extracellular Mg2+. In addition, they could be blocked by physiological concentrations of intracellular Ca2+. In accordance with these electrophysiological results, analysis of mRNA revealed the expression of a gene coding for h-eag1 channels in IGR1 and IPC298 cells, but not in IGR39 cells. At elevated Ca2+ concentrations various types of Ca2+-activated K+ channels with single-channel characteristics similar to IK and SK channels were detected in IGR1 cells. The whole-cell Ca2+ activated K+ currents were not voltage dependent, insensitive for 100 nM apamin and 200 mu M d-tubocurarine, but were blocked by charybdotoxin (100 nM) and clotrimazole (50 nM). Analysis of mRNA revealed the expression of hSK1, hSK2, and hIK channels in IGR1 cells.
引用
收藏
页码:107 / 115
页数:9
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