The vaccinia virus B9R protein is a 6 kDa intracellular protein that is non-essential for virus replication and virulence

被引:12
作者
Price, N [1 ]
Tscharke, DC [1 ]
Smith, GL [1 ]
机构
[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England
关键词
D O I
10.1099/0022-1317-83-4-873
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Vaccinia virus (VV) strain Western Reserve gene B9R is shown to encode an intracellular 6 kDa protein that is expressed late during the infectious cycle. In vitro transcription and translation produced two polypeptides in the presence of microsomal membranes, but only the larger protein in the absence of membranes. The smaller protein sedimented with microsomes during centrifugation, suggesting it was inserted into the lipid membrane or into the microsomal lumen via the N-terminal hydrophobic signal sequence that was subsequently cleaved proteolytically. A VV mutant lacking B9R was constructed and found to replicate normally in cell culture and two in vivo models.
引用
收藏
页码:873 / 878
页数:6
相关论文
共 27 条
[1]   NUCLEOTIDE-SEQUENCE OF 21.8 KBP OF VARIOLA MAJOR VIRUS-STRAIN HARVEY AND COMPARISON WITH VACCINIA VIRUS [J].
AGUADO, B ;
SELMES, IP ;
SMITH, GL .
JOURNAL OF GENERAL VIROLOGY, 1992, 73 :2887-2902
[2]  
ALCAMI A, 1995, J VIROL, V69, P4633
[3]   A SOLUBLE RECEPTOR FOR INTERLEUKIN-1-BETA ENCODED BY VACCINIA VIRUS - A NOVEL MECHANISM OF VIRUS MODULATION OF THE HOST RESPONSE TO INFECTION [J].
ALCAMI, A ;
SMITH, GL .
CELL, 1992, 71 (01) :153-167
[4]   The complete genomic sequence of the modified vaccinia Ankara strain: Comparison with other orthopoxviruses [J].
Antoine, G ;
Scheiflinger, F ;
Dorner, F ;
Falkner, FG .
VIROLOGY, 1998, 244 (02) :365-396
[5]   ESCHERICHIA-COLI GPT GENE PROVIDES DOMINANT SELECTION FOR VACCINIA VIRUS OPEN READING FRAME EXPRESSION VECTORS [J].
FALKNER, FG ;
MOSS, B .
JOURNAL OF VIROLOGY, 1988, 62 (06) :1849-1854
[6]   TRANSIENT DOMINANT SELECTION OF RECOMBINANT VACCINIA VIRUSES [J].
FALKNER, FG ;
MOSS, B .
JOURNAL OF VIROLOGY, 1990, 64 (06) :3108-3111
[7]   A CAPRIPOXVIRUS PSEUDOGENE WHOSE ONLY INTACT HOMOLOGS ARE IN OTHER POXVIRUS GENOMES [J].
GERSHON, PD ;
BLACK, DN .
VIROLOGY, 1989, 172 (01) :350-354
[8]   THE COMPLETE DNA-SEQUENCE OF VACCINIA VIRUS [J].
GOEBEL, SJ ;
JOHNSON, GP ;
PERKUS, ME ;
DAVIS, SW ;
WINSLOW, JP ;
PAOLETTI, E .
VIROLOGY, 1990, 179 (01) :247-266
[9]  
HORTON RM, 1990, BIOTECHNIQUES, V8, P528
[10]   VACCINIA VIRUS HOMOLOGS OF THE SHOPE FIBROMA VIRUS INVERTED TERMINAL REPEAT PROTEINS AND A DISCONTINUOUS ORF RELATED TO THE TUMOR-NECROSIS-FACTOR RECEPTOR FAMILY [J].
HOWARD, ST ;
CHAN, YS ;
SMITH, GL .
VIROLOGY, 1991, 180 (02) :633-647