Cloning, expression and chromosome localization of porcine adiponectin and adiponectin receptors genes

被引：34

作者：

Dai, MH ^{[1
]}

Xia, T ^{[1
]}

Zhang, GD ^{[1
]}

Chen, XD ^{[1
]}

Gan, L ^{[1
]}

Feng, SQ ^{[1
]}

Qiu, H ^{[1
]}

Peng, Y ^{[1
]}

Yang, ZQ ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Life Sci & Technol, Biochem Lab, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

来源：

DOMESTIC ANIMAL ENDOCRINOLOGY | 2006年 / 30卷 / 02期

基金：

中国国家自然科学基金;

关键词：

pigs; adiponectin; adiponectin receptors; chromosomal localization; tissue distribution;

D O I：

10.1016/j.domaniend.2005.06.006

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Adiponectin is a cytokine secreted specifically by adipocytes that has been proposed to enhance insulin sensitivity and prevent atherosclerosis. Adiponectin receptors (adipoR1 and adipoR2) are recently found in mice which act as receptors for globular and full-length adiponectin to mediate the fatty-acid oxidation and glucose uptake ill Muscle and liver. The primary goal of this study was to examine chromosome localization of porcine adiponectin and adiponectin receptors and the gene expression pattern ill Various tissues of pigs of the three genes. Radiation hybrid mapping demonstrated that porcine adiponectin, adipoR1 and adipoR2 were located to chromosome 13q36-41, 10p11 and 5q25, in the regions that were syntenic to the homologs of human genes, respectively. Semi-quantitative RT-PCR showed that porcine adiponectin mRNA was specifically expressed in adipose tissue and porcine adipoR1 and adipoR2 mRNA were ubiquitously expressed in many tissues except brain. Comparison to adipoR2 mRNA which was highly expressed in fiver, heart, kidney, adipose issues and lung, adipoR1 mRNA was expressed at relatively high levels in porcine muscle, leukocytes and epididymis. Our data provide basic molecular information useful for the further investigation oil the function of the three genes. (c) 2005 Elsevier Inc. All rights, reserved.

引用

页码：117 / 125

页数：9

共 24 条

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