Proteomics: analytical tools and techniques

被引:32
作者
MacCoss, MJ [1 ]
Yates, JR [1 ]
机构
[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92122 USA
关键词
D O I
10.1097/00075197-200109000-00006
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Scientists have long been interested in measuring the effects of different stimuli on protein expression and metabolism. Analytical methods are being developed for the automated separation, identification, and quantitation of all of the proteins within the cell. Soon, investigators will be able to observe the effects of an experiment on every protein (as opposed to a selected few). This review presents a discussion of recent technological advances in proteomics in addition to exploring current methodological limitations. Curr Opin Clin Nutr Metab Care 4:369-375, (C) 2001 Lippincott Williams Wilkins.
引用
收藏
页码:369 / 375
页数:7
相关论文
共 27 条
[21]   Immobilized gallium(III) affinity chromatography of phosphopeptides [J].
Posewitz, MC ;
Tempst, P .
ANALYTICAL CHEMISTRY, 1999, 71 (14) :2883-2892
[22]   Large-scale analysis of the yeast proteome by multidimensional protein identification technology [J].
Washburn, MP ;
Wolters, D ;
Yates, JR .
NATURE BIOTECHNOLOGY, 2001, 19 (03) :242-247
[23]  
WATTS JD, 1994, J BIOL CHEM, V269, P29520
[24]  
Weckwerth W, 2000, RAPID COMMUN MASS SP, V14, P1677, DOI 10.1002/1097-0231(20000930)14:18<1677::AID-RCM84>3.0.CO
[25]  
2-N
[26]   A systematic approach to the analysis of protein phosphorylation [J].
Zhou, HL ;
Watts, JD ;
Aebersold, R .
NATURE BIOTECHNOLOGY, 2001, 19 (04) :375-378
[27]   Detection and sequencing of phosphopeptides affinity bound to immobilized metal ion beads by matrix-assisted laser desorption/ionization mass spectrometry [J].
Zhou, W ;
Merrick, BA ;
Khaledi, MG ;
Tomer, KB .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2000, 11 (04) :273-282