Study of a rat skin in vivo micronucleus test: data generated by mitomycin C and methyl methanesulfonate

被引：32

作者：

Nishikawa, T

Haresaku, M

Adachi, K

Masuda, M

Hayashi, M

机构：

[1] Lion, Human Safety Evaluat Ctr, Odawara, Kanagawa 2560811, Japan

[2] Lion, Life Sci Res Ctr, Odawara, Kanagawa 2560811, Japan

[3] Natl Inst Hlth Sci, Div Genet & Mutagenesis, Setagaya Ku, Tokyo 1585801, Japan

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 1999年 / 444卷 / 01期

关键词：

skin; genotoxicity; micronucleus test; in vivo assay; mitomycin C; methyl methanesulfonate;

D O I：

10.1016/S1383-5718(99)00084-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed an in vivo micronucleus (MN) test that uses rat skin as the target organ. Sample preparation involves cold-treating the epidermis with trypsin, peeling it off with a fine forceps, treating it in hypotonic solution, and staining it with acridine orange (A.O.). We evaluated the assay using mitomycin C (MMC) and methyl methanesulfonate (MMS) as model clastogens, applying them as single and repeat treatments. Both chemicals induced a significant, dose-dependent increase in MN frequency in basal cells. One treatment per day for 3 days was optimal for MN induction. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：159 / 166

页数：8

共 23 条

[1] TUMORIGENICITY OF CYCLOPENTA[A]PHENANTHRENE DERIVATIVES AND MICRONUCLEUS INDUCTION IN MOUSE SKIN [J].