Novel intravenous 13C-methionine breath test as a measure of liver function in children with short bowel syndrome

Monitoring hepatic function in children with short bowel syndrome (SBS) and parenteral nutrition-associated liver disease (PNALD) is currently limited to conventional blood testing or liver biopsy. Metabolism of the stable isotope L[1-C-13]methionine occurs exclusively in liver mitochondria and can be noninvasively quantified in expired breath samples. We hypothesized that the, 3 C-methionine breath test (C-13-MBT) could be a safe, noninvasive, and valid measure of hepatic mitochondrial function in children with SBS and PNALD. Methods: Baseline breath samples were collected in 8 children with SBS before intravenous administration of 2 mg/kg of L[1-C-13]methionine. Six paired breath samples were obtained at 20-minute intervals. The (CO2)-C-13, enrichment was analyzed using isotope ratio mass spectrometry. Results: All 8 patients (5 males;, mean age, 8.9 months) tolerated the C-13-MIBT without adverse events. Two patients underwent serial testing. One patient, tested before and after resolution of cholestasis, demonstrated increased Cumulative percentage dose (4.7% to 6.6%) and area under the curve (AUC) (270-303). A second patient with progressive PNALD demonstrated decreased cumulative percentage dose (from 7.8% to 5.9%) and AUC (from 335 to 288). Conclusion: The C-13-MBT is a feasible, safe, and potentially clinically relevant measure of hepatic mitochondrial function in children with SBS and PNALD. (c) 2009 Elsevier Inc. All rights reserved.