The noncatalytic site-deficient α3β3γ subcomplex and F0F1-ATP synthase can continuously catalyse ATP hydrolysis when Pi is present

被引：13

作者：

Bald, D ^{[1
]}

Muneyuki, E ^{[1
]}

Amano, T ^{[1
]}

Kruip, J ^{[1
]}

Hisabori, T ^{[1
]}

Yoshida, M ^{[1
]}

机构：

[1] Tokyo Inst Technol, Resources Utilizat Res Lab, Midori Ku, Yokohama, Kanagawa 226, Japan

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1999年 / 262卷 / 02期

关键词：

ATP synthase; MgADP inhibited form; noncatalytic nucleotide binding sites; P-i;

D O I：

10.1046/j.1432-1327.1999.00410.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated ATP hydrolysis by a mutant (Delta NC) alpha(3)beta(3)gamma subcomplex of F0F1-ATP synthase from the thermophilic Bacillus PS3 that is defective in the noncatalytic nucleotide binding sites. This mutant subcomplex was activated by inorganic phosphate ions (P-i) and did not show continuous ATP hydrolysis activity in the absence of P-i. P-i also activated the wild-type alpha(3)beta(3)gamma subcomplex in a similar manner. Sulphate activated wild-type alpha(3)beta(3)gamma but not Delta NC alpha(3)beta(3)gamma, indicating that P-i activation did not involve noncatalytic sites but that sulphate activation did. P-i also activated ATP hydrolysis and coupled proton translocation by the wild-type and Delta NC F0F1-ATP synthases reconstituted into vesicle membranes.

引用

页码：563 / 568

页数：6

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