Molecular cloning and characterization of MSRV-related sequences associated with retrovirus-like particles

被引：99

作者：

Komurian-Pradel, F

Paranhos-Baccala, G

Bedin, F

Ounanian-Paraz, A

Sodoyer, M

Ott, C

Rajoharison, A

Garcia, E

Mallet, F

Mandrand, B

Perron, H

机构：

[1] Ecole Normale Super Lyon, CNRS BioMerieux, UMR 103, F-69364 Lyon 07, France

[2] Univ Grenoble 1, Fac Med, Virol Lab, F-38700 Grenoble, France

来源：

VIROLOGY | 1999年 / 260卷 / 01期

关键词：

D O I：

10.1006/viro.1999.9792

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

New sequences have been obtained by successive overlapping RT-PCR extensions from the pol region of a retroviral RNA (multiple sclerosis-associated retroviral element, MSRV) amplified in retrovirus-like particles from patients with multiple sclerosis. gag and pol sequences are related to type C oncoviruses, whereas the env sequence is closer to type D. A tryptophan-like (W) tRNA primer-binding site was identified downstream of the RU5 region in the 5'LTR, and the U3R region cloned in the 3'LTR exhibited potent promoter activity. MSRV clones define a novel family of endogenous elements, HERV-W. From our data, HERV-W RNAs are copackaged in extracellular particles which might be produced by replication-competent or transcomplemented HERV-W copies or by an exogenous member of the HERV-W family. (C) 1999 Academic Press.

引用

页码：1 / 9

页数：9

共 20 条

[1] Unique long terminal repeat U3 sequences distinguish exogenous jaagsiekte sheep retroviruses associated with ovine pulmonary carcinoma from endogenous loci in the sheep genome [J].