Creation of biomolecule arrays by electrostatic immobilization on electron-beam-irradiated polystyrene thin films

被引：20

作者：

Wybourne, MN ^{[1
]}

Yan, MD ^{[1
]}

Keana, JFW ^{[1
]}

Wu, JC ^{[1
]}

机构：

[1] UNIV OREGON,DEPT CHEM,EUGENE,OR 97403

来源：

NANOTECHNOLOGY | 1996年 / 7卷 / 03期

关键词：

D O I：

10.1088/0957-4484/7/3/021

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Thin polystyrene films irradiated by an electron beam to create trapped charge are shown to immobilize the positively-charged protein, avidin-fluorescein. Using electron-beam lithography, fluorescent patterns with a minimum feature size of 0.5 mu m were formed. The electrostatic nature of the immobilization was supported by the spatial and temporal features of the immobilization process. Additional evidence for an electrostatic mechanism was obtained from experiments to screen the trapped charge density with poly-L-lysine.

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页码：302 / 305

页数：4

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