Effects of acute exposure to aluminum on blood-brain barrier and the protection of zinc

被引：45

作者：

Song, Yang ^{[2
]}

Xue, Yixue ^{[1
]}

Liu, Xin ^{[1
]}

Wang, Ping ^{[1
]}

Liu, Libo ^{[1
]}

机构：

[1] China Med Univ, Coll Basic Med, Dept Neurobiol, Shenyang 110001, Peoples R China

[2] China Med Univ, Coll Basic Med, Ctr Funct Subjects, Dept Expt, Shenyang 110001, Peoples R China

来源：

NEUROSCIENCE LETTERS | 2008年 / 445卷 / 01期

关键词：

Aluminum; Zinc; Blood-brain barrier; F-actin; Occludin;

D O I：

10.1016/j.neulet.2008.08.081

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Aluminum and zinc are two important trace elements in an organism. Although several studies have demonstrated their impacts on the intelligence, very little was known about their effects on the integrity of blood-brain barrier (BBB). To study the effects of aluminum and zinc on the permeability of BIBB, different doses of aluminum and appropriate zinc were administered to rats. Evans blue was detected in brain to determine the permeability of BBB. The ultrastructure of BBB was observed under the transmission electron Microscope. Immunohistochemistry and Western blot method were used to detect the expression of skeleton protein F-actin and tight junction protein occludin in brain capillary endothelium. The data indicated that compared with the control group, Evans blue in brains increased (P<0.01), the ultrastructure of BBB changed and the expression of F-actin and occludin decreased (P<0.01) in the aluminum-toxic group. Compared with the aluminum-toxic groups, the permeability of BBB to Evans blue decreased (P<0.01), the damage of the BBB ultrastructure was attenuated and the expression off-actin and occludin increased (P<0.05) in the aluminum-zinc group. Our present studies suggest that aluminum increases the permeability of BBB by changing its ultrastructure and the expression of occludin and F-actin. Zinc can protect the integrity of BBB in juvenile Fats that are exposed to aluminum and inhibit the decrease of tight junction protein occludin and F-actin expression in BBB. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：42 / 46

页数：5

共 29 条

[1] Aluminium and Alzheimer's disease: Review of possible pathogenic mechanisms [J].

Armstrong, RA ;

Winsper, SJ ;

Blair, JA .

DEMENTIA, 1996, 7 (01) :1-9

[2]

Bamforth SD, 1999, J CELL SCI, V112, P1879

[3]

Constantinidis J, 1990, Schweiz Arch Neurol Psychiatr (1985), V141, P523

[4] Divergent effects of zinc depletion in brain vs non-brain endothelial cells [J].

Di Cello, F ;

Siddharthan, V ;

Paul-Satyaseela, M ;

Kim, KS .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 335 (02) :373-376

[5] Aluminium as a risk factor in Alzheimer's disease, with emphasis on drinking water [J].

Flaten, TP .

BRAIN RESEARCH BULLETIN, 2001, 55 (02) :187-196

[6]

GRATTAROLA M, 1982, BASIC APPL HISTOCHEM, V26, P153

[7] Dexamethasone enhances adenosine 5′-triphosphate-sensitive potassium channel expression in the blood-brain tumor barrier in a rat brain tumor model [J].

Gu, Yan-ting ;

Zhang, Hua ;

Xue, Yi-xue .

BRAIN RESEARCH, 2007, 1162 :1-8

[8] Aluminum exposure and Alzheimer's disease [J].

Jansson, Erik T. .

JOURNAL OF ALZHEIMERS DISEASE, 2001, 3 (06) :541-549

[9] Memory deficit in mice administered aluminum-maltolate complex [J].

Kaneko, N ;

Takada, J ;

Yasui, H ;

Sakurai, H .

BIOMETALS, 2006, 19 (01) :83-89

[10] Effects of aluminum on the neurotoxicity of primary cultured neurons and on the aggregation of β-amyloid protein [J].

Kawahara, M ;

Kato, M ;

Kuroda, Y .

BRAIN RESEARCH BULLETIN, 2001, 55 (02) :211-217

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