Application of random amplified polymorphic DNA (RAPD) analysis for identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets

被引：29

作者：

Asensio, L ^{[1
]}

González, I ^{[1
]}

Fernández, A ^{[1
]}

Rodríguez, MA ^{[1
]}

Lobo, E ^{[1
]}

Hernández, PE ^{[1
]}

García, T ^{[1
]}

Martín, R ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr & Bromatol Higiene & Tecnol Alimentos 3, E-28040 Madrid, Spain

来源：

JOURNAL OF FOOD PROTECTION | 2002年 / 65卷 / 02期

关键词：

D O I：

10.4315/0362-028X-65.2.432

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A random amplified polymorphic DNA (RAPD) method was developed for the specific identification of grouper, (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. Using two different reaction primers (S1 and L1), RAPD analysis produced clear fingerprints from which the three fish species could be easily identified. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.

引用

页码：432 / 435

页数：4

共 25 条

[1] Identification of Nile perch (Lates niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus) by polymerase chain reaction-restriction fragment length polymorphism of a 12S rRNA gene fragment [J].