Evidence from CD spectra and melting temperatures for stable Hoogsteen-paired oligomer duplexes derived from DNA and hybrid triplexes

被引：26

作者：

Hashem, GM ^{[1
]}

Wen, JD ^{[1
]}

Do, Q ^{[1
]}

Gray, DM ^{[1
]}

机构：

[1] Univ Texas, Dept Cell & Mol Biol, Richardson, TX 75083 USA

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 16期

关键词：

D O I：

10.1093/nar/27.16.3371

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pyr*pur.pyr type of nucleic acid tripler has a purine strand that is Hoogsteen-paired with a parallel pyrimidine strand (pyr*pur pair) and that is Watson-Crick-paired with an antiparallel pyrimidine strand (pur.pyr pair). In most cases, the Watson-Crick pair is more stable than the Hoogsteen pair, although stable formation of DNA Hoogsteen-paired duplexes has been reported. Using oligomer triplexes of repeating d(AG)(12) and d(CT)(12) or r(CU)(12) sequences that were 24 nt long, we found that hybrid RNA*DNA as well as DNA*DNA Hoogsteen-paired strands of triplexes can be more stable than the Watson-Crick-paired strands at low pH. The structures and relative stabilities of these duplexes and triplexes were evaluated by circular dichroism (CD) spectroscopy and UV absorption melting studies of triplexes as a function of pH. The CD contributions of Hoogsteen-paired RNA*DNA and DNA*DNA duplexes were found to dominate the CD spectra of the corresponding pyr*pur.pyr triplexes.

引用

页码：3371 / 3379

页数：9

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